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Species: Drosophila melanogaster
Genetic Insert: micro-RNA 315
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22765 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16177138
Proper citation: RRID:Addgene_22760 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 13b-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22762 Copy
Species: Drosophila melanogaster
Genetic Insert: micro-RNA-92b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17901217
Proper citation: RRID:Addgene_22709 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 6-1, micro RNA 6-2, micro RNA 6-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22789 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 9b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17901217
Proper citation: RRID:Addgene_22710 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17901217
Proper citation: RRID:Addgene_22711 Copy
Species: Drosophila melanogaster
Genetic Insert: Mad
Vector Backbone Description: Backbone Size:4096; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657393
Proper citation: RRID:Addgene_22996 Copy
Species: Drosophila melanogaster
Genetic Insert: Mad
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657393
Proper citation: RRID:Addgene_22997 Copy
Species: Drosophila melanogaster
Genetic Insert: p174
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression, Stratagene; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_25889 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:8033; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26224 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:7904; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26222 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Proper citation: RRID:Addgene_26859 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26856 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26855 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. Myc-tagged STOPS lacking the SOCS box was
subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26857 Copy
Species: Drosophila melanogaster
Genetic Insert: no receptor potential A
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX
Proper citation: RRID:Addgene_26854 Copy
Species: Drosophila melanogaster
Genetic Insert: Pavarotti
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4200; Vector Backbone:pMT-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19720876
Comments: This plasmid was originally generated by Dr. Gohta Goshima and published in MBOC. Vol. 16, 3896–3907.
Proper citation: RRID:Addgene_24286 Copy
Species: Drosophila melanogaster
Genetic Insert: p150 Dynactin
Vector Backbone Description: Backbone Marker:Drosophila Gateway Vector Collection; Backbone Size:5943; Vector Backbone:pMT-GW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17576797
Comments: Dr. Goshima made a C-terminal GFP p150 plasmid and Vale lab used his pENTR vector to make an N-terminal fusion using Gateway cloning.
Y1238H mutation detected in Addgene's BGH-rev sequence is not likely to affect function.
Proper citation: RRID:Addgene_24283 Copy
Species: Drosophila melanogaster
Genetic Insert: Shibire (ts1)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: Shibire was PCR amplified from genomic DNA of UAS-shibire transgenic flies (Kitamoto, 2001) using genUASFOR and genUASREVsv40 oligos, and ligated into the NotI/KpnI sites of pQUAST.
Proper citation: RRID:Addgene_24362 Copy
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