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Species: Synthetic
Genetic Insert: 6xHIS-GB1
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174587 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174495 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27473911
Comments: For protein expression, grow at 17degC.
Plasmid used in the following publication:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
Proper citation: RRID:Addgene_174496 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN6
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174499 Copy
Vector Backbone Description: Backbone Size:4506; Vector Backbone:pKO403; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35023783
Proper citation: RRID:Addgene_174726 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174766 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174768 Copy
Species: N. benthamiana
Genetic Insert: GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174762 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-D481V with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174763 Copy
Species: Synthetic
Genetic Insert: Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172150 Copy
Species: Synthetic
Genetic Insert: pCDF.B.(SD4)CD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172146 Copy
Species: Aequorea victoria
Genetic Insert: CDS C-terminal eYFP (175-238)
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172779 Copy
Species: Arabidopsis thaliana
Genetic Insert: CDS of chloroplast outer envelope membrane protein OEP7
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172776 Copy
Species: Escherichia coli
Genetic Insert: TurboID with 3XHA tag
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172795 Copy
Species: Aequorea victoria
Genetic Insert: Spacer+NtVenus 1-210 STOP pAGM1301 (CT)
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM130; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172785 Copy
Species: Aequorea victoria
Genetic Insert: CDS 3XFLAG tag N-terminal eYFP (1-174)
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172781 Copy
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34190535
Comments: pIT5_SL is designed to be integrated into the lambda attachment site, using the lambda integrase helper plasmid pInt-ts (Addgene # 66076).
Proper citation: RRID:Addgene_173769 Copy
Species: Rippkaea orientalis
Genetic Insert: partial tRNA-Leugene from Rippkaea orientalis
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178774 Copy
Species: Rippkaea orientalis
Genetic Insert: Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178771 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2868; Vector Backbone:BASIC_SEVA_47_pKD46.10; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178932 Copy
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