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Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36384003
Proper citation: RRID:Addgene_205225 Copy
Vector Backbone Description: Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome.
Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates.
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220280 Copy
Species: Photinus pyralis
Genetic Insert: ffluc
Vector Backbone Description: Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details.
Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication:
Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability
Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier
bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891
The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900).
Please download the detailed plasmid map using the file linked below
Proper citation: RRID:Addgene_218513 Copy
Vector Backbone Description: Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220248 Copy
Species: Synthetic
Genetic Insert: HR1b
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1178
Proper citation: RRID:Addgene_211853 Copy
Species: Salmonella typhimurium
Genetic Insert: LRR-NEL
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38446628
Comments: Rojas-Pierce Lab plasmid pMRP1137
Proper citation: RRID:Addgene_211862 Copy
Species: Synthetic
Genetic Insert: Connector R11
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216437 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter eno101
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216438 Copy
Species: Synthetic
Genetic Insert: Connector R7
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216433 Copy
Species: Synthetic
Genetic Insert: Connector R6
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216432 Copy
Species: Synthetic
Genetic Insert: Connector R8
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216434 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter tif51
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216442 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter rpl2501
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216441 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter rpl2102
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216444 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter rpl701
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216443 Copy
Species: Synthetic
Genetic Insert: Connector L6
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216426 Copy
Species: Synthetic
Genetic Insert: Connector L9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216429 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter apl4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216448 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter tub1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216446 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Promoter nmt1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37991801
Proper citation: RRID:Addgene_216451 Copy
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