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Species: Chryseobacterium sp. JM1
Genetic Insert: ChrH
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41911463
Comments: Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.
Proper citation: RRID:Addgene_257760 Copy
Species: Melainabacteria bacterium isolate SJ512
Genetic Insert: MelH
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41911463
Comments: Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.
Proper citation: RRID:Addgene_257765 Copy
Species: Hymenobacter gelipurpurascens DSM 11116
Genetic Insert: HymH
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41911463
Comments: Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.
Proper citation: RRID:Addgene_257763 Copy
Species: Neisseria dentiae CCUG 53898 310003
Genetic Insert: NedB and NedC
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41911463
Comments: Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.
Proper citation: RRID:Addgene_257772 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sc RFC5 (mutant)
Vector Backbone Description: Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.
Proper citation: RRID:Addgene_258210 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5′ CYSTM α (From the 5' UTR of CYSTM1 gene)
Vector Backbone Description: Backbone Size:14340; Vector Backbone:pCAMBIA; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:42194977
Comments: Please visit https://doi.org/10.1101/2025.10.15.682649 for bioRxiv preprint.
Proper citation: RRID:Addgene_247493 Copy
Species: Melainabacteria bacterium isolate SJ512
Genetic Insert: His-MelH and MelHc
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41911463
Comments: Please visit https://doi.org/10.1101/2025.10.31.685832 for bioRxiv preprint.
Proper citation: RRID:Addgene_257767 Copy
Species: Parageobacillus thermoglucosidasius
Genetic Insert: adhT
Vector Backbone Description: Vector Backbone:pZASS; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40245979
Comments: Please visit https://doi.org/10.1101/2024.10.31.621308 for bioRxiv preprint.
Proper citation: RRID:Addgene_231854 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Removal of Anthoceros agrestis RbcX2 and RbcX1 from pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1 was performed by Genscript.
Proper citation: RRID:Addgene_229515 Copy
Species: Arabidopsis thaliana
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Design of the Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221), with the addition of RbcX isoform, RbcXI after the BSD2 gene.
Proper citation: RRID:Addgene_229510 Copy
Species: Arabidopsis thaliana
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Design of the three Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221).
Proper citation: RRID:Addgene_229511 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Removal of Anthoceros agrestis Raf2 from pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1 was performed by Genscript.
Proper citation: RRID:Addgene_229513 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229507 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Each assembly factor is regulated by a individual T7 promoter and terminator.
Proper citation: RRID:Addgene_229509 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229508 Copy
Vector Backbone Description: Backbone Size:5261; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39126322
Proper citation: RRID:Addgene_213132 Copy
Species: Chryseobacterium sp. JM1
Genetic Insert: ChrH
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:37252350
Comments: Also encodes ChrI (no tag)
Proper citation: RRID:Addgene_225083 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216749 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216750 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216752 Copy
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