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Species: Rattus norvegicus
Genetic Insert: PV-NES-GFP
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971908
Proper citation: RRID:Addgene_17301 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17331 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged2
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17332 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6100; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The backbone of this plasmid appears to be approximately 600bp smaller than is reported. The depositing scientist is aware of this difference and reports that the plasmid functions as it should.
Proper citation: RRID:Addgene_17336 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKII-T286D
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777564
Comments: Description: Constitutively active CAMKII (threonine 286 mutated to aspartate)
provided by H. Schulman (Vanderbilt) subcloned into the EcoRI site of CS2+.
Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
Proper citation: RRID:Addgene_16736 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(K42M)-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167477 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(T286A)-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167476 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167475 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1-HA-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Comments: Rfz1 from Henk Roelink. This sequence has a few differences.
from the published sequence. Sites listed in Rfz1 are not a complete list.
and I haven't searched through the CS2+ sequence to see if/where those sites.
are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope.
into the StyI site (1904). The 3' Sty site is destroyed by the tags, but.
the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed.
tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample.
buffer when boiled, so either warm samples to ~70 degrees Celsius for a.
few minutes before loading samples or use a strong denaturing loading buffer.
and incubate the samples at room temp for half and hour before loading.
The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16797 Copy
Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-2 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
Proper citation: RRID:Addgene_16794 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1, and GFP at the C terminus.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16815 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The the insert was published by Wang et al., JBC 271: 4468 (1996), and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16813 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA, tagged with a single epitope in the third extracellular loop (at an introduced NheI site) was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16812 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA, tagged with a c-myc epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16819 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA, tagged with an HA epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The HA-tag insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16818 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of rat Frizzled 1 with a carboxy terminal GFP tag.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16817 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Notes for Use: Construct is 8251bp, made 9/16/02 by Ajamete, and
also called Fz1-ER/AKA pC4FZ1agg.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16816 Copy
Species: Rattus norvegicus
Genetic Insert: rat LAMP1-mRFP
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33296658
Proper citation: RRID:Addgene_169004 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27818143
Proper citation: RRID:Addgene_168997 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Vector Backbone:pT2AL200R150G; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27818143
Proper citation: RRID:Addgene_168998 Copy
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