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Species: polyomavirus
Genetic Insert: VP1 from Human Polyomavirus 7
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 22517; Backbone Size:5388; Vector Backbone:pGwf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:20542254
Proper citation: RRID:Addgene_24726 Copy
Species: polyomavirus
Genetic Insert: Full genome of Human polyomavirus 7
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:20542254
Comments: genome can be liberated by digestion with Mfe I.
Proper citation: RRID:Addgene_24728 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4578; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19680558
Comments: Bigenic vector with EF-1alpha promoter and CMV promoter
Proper citation: RRID:Addgene_23027 Copy
Genetic Insert: Firefly luciferase shRNA
Vector Backbone Description: Backbone Size:5596; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_21561 Copy
Genetic Insert: sh Ataxia telangiectasia mutated
Vector Backbone Description: Backbone Size:5540; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Comments: shATM #2 was used successfully in Rodier et al.(2009) Nat.Cell Biol. 11(8):973-979.
Proper citation: RRID:Addgene_22528 Copy
Species: Murine Polyomavirus
Genetic Insert: MPyV VP2
Vector Backbone Description: Backbone Size:3973; Vector Backbone:phGf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19750217
Comments: First reference to this plasmid was in:
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6
Genbank style annotations can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm
Proper citation: RRID:Addgene_22520 Copy
Genetic Insert: sh Enhanced Green Fluorescent Protein #1
Vector Backbone Description: Backbone Size:5596; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_22530 Copy
Species: Merkel Cell Polyomavirus
Genetic Insert: MCPyV VP1
Vector Backbone Description: Backbone Size:5338; Vector Backbone:pGwf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19750217
Comments: First reference to this plasmid was in:
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6
Genbank style annotations can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm
Proper citation: RRID:Addgene_22515 Copy
Species: Mus musculus
Genetic Insert: Intercellular Adhesion Molecule 1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4179; Vector Backbone:pFUSE; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32783949
Comments: Depositor confirms P66S and L234P mutations does not affect plasmid function.
Proper citation: RRID:Addgene_156462 Copy
Species: Synthetic
Genetic Insert: pMD2.G(spei/spei)&12x601_tetO
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR™BluntII-TOPO®; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31543265
Comments: High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157785 Copy
Genetic Insert: PGL3 luciferase siRNA
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs
See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case.
Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.
Proper citation: RRID:Addgene_15099 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
Proper citation: RRID:Addgene_15098 Copy
Species: Mus musculus
Genetic Insert: Kir3.2
Vector Backbone Description: Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32372643
Proper citation: RRID:Addgene_140745 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158719 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158720 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33093202
Comments: Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
Proper citation: RRID:Addgene_159762 Copy
Species: Homo sapiens
Genetic Insert: HPV16L1+ GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:many different ones; Vector Types:Unspecified; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15709003
Proper citation: RRID:Addgene_89910 Copy
Species: Homo sapiens
Genetic Insert: Anti-Ebola 2G4 monoclonal antibody
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:28786662
Proper citation: RRID:Addgene_85933 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRIC-B1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3221; Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27698420
Comments: Vector pPICZ A and insert sequence were modified as follows:
1. Restriction sites including Pml I; Sfi I; BsmB I; Asp718 I; Kpn I were destroyed during cloning full-length CeTRIC-B1 to the vector.
2. In the C-terminal truncation, the sequence between XhoI and polyhistidine had been deleted. That is to say, restriction sites including Xho I, Sac II, Not I, Apa I, and myc-epitope, were destroyed during truncation.
3. A kozak suqence "ACC" was inserted between the restriction site "EcoR I" and initial codon, we added these 3 bp into insert sequence size.
In summary, the size of pPICZ A backbone is changed from 3329 bp to be 3221 bp.
Proper citation: RRID:Addgene_87344 Copy
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