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Species: Homo sapiens
Genetic Insert: WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: wild-type tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. Wild-type tRNA has high pol-III promoter activity with high processing. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124541 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124540 Copy
Species: Homo sapiens
Genetic Insert: deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124543 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124545 Copy
Species: Homo sapiens
Genetic Insert: deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124544 Copy
Species: Mus musculus
Genetic Insert: p65 promoter- p65 CDS-Clover
Vector Backbone Description: Backbone Size:7475; Vector Backbone:pLenti _Puro_DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396000
Proper citation: RRID:Addgene_124549 Copy
Species: Homo sapiens
Genetic Insert: deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124539 Copy
Species: Mus musculus
Genetic Insert: C1V1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124650 Copy
Species: Homo sapiens
Genetic Insert: c-Myc Renilla reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124532 Copy
Species: Homo sapiens
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124652 Copy
Species: Homo sapiens
Genetic Insert: P53 NLuc reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124526; Backbone Size:2328; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124533 Copy
Species: Homo sapiens
Genetic Insert: FOXO Renilla reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124535 Copy
Species: Homo sapiens
Genetic Insert: deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124537 Copy
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:5000; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32539183
Comments: Destination vector. BsmBI cloning site. Pink/White selection
Proper citation: RRID:Addgene_124528 Copy
Species: Halorubrum sp. TP009
Genetic Insert: ArchT
Vector Backbone Description: Backbone Marker:Karl Deisseroth (EF1a-fDIO-hChr2(H134R)-EYFP, Addgene 55639); Backbone Size:5603; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Flp/Frt; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30773318
Proper citation: RRID:Addgene_124640 Copy
Species: Homo sapiens
Genetic Insert: RGECO-TnT
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7500; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30732532
Proper citation: RRID:Addgene_124643 Copy
Species: Azotobacter vinelandii
Genetic Insert: Flavodoxin II
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5403; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28710816
Proper citation: RRID:Addgene_124520 Copy
Species: S. pyogenes
Genetic Insert: NL-DHFR-SpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:4000; Vector Backbone:pX330; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30834641
Proper citation: RRID:Addgene_124522 Copy
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:2334; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124525 Copy
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:2330; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124527 Copy
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