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Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38844479
Proper citation: RRID:Addgene_227000 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5262; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201000 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5565; Vector Backbone:pTn7-SCOUT22; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201019 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5262; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200992 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5565; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200996 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_211942 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:13909; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_216394 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_211941 Copy
Genetic Insert: Yellow fluorescent protein
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pMB1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33443634
Proper citation: RRID:Addgene_215654 Copy
Species: Homo sapiens
Genetic Insert: WapI
Vector Backbone Description: Backbone Size:3913; Vector Backbone:pCR2.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37357575
Proper citation: RRID:Addgene_217666 Copy
Genetic Insert: the groESL region region from E. coli is the insert in the pBR325 plasmid pOF39
Vector Backbone Description: Vector Backbone:the pOF39 plasmid backbone is pBR325; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36973666
Comments: Please see the BM28 genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102380) and the accompanying papers for more details.
Additional publications:
Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180.
And for the pOF39 plasmid: Fayet O, Louarn JM, Georgopoulos C. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes. Mol Gen Genet. 1986 Mar;202(3):435-45. doi: 10.1007/BF00333274. PMID: 3012269.
Proper citation: RRID:Addgene_201047 Copy
Species: Homo sapiens
Genetic Insert: 2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
Vector Backbone Description: Vector Backbone:AAVS1-SA-Puro; Vector Types:ZNF donor; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36989136
Comments: For this purpose, a previously generated backbone containing 2xCHS4-EF1a-tdTomato-SV40-2xCHS4 was used (Bagley et al., 2017).
Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_196192 Copy
Species: Homo sapiens
Genetic Insert: trLAT
Vector Backbone Description: Backbone Marker:Ilya Levental lab; Vector Backbone:trLAT-mRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203745 Copy
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:4812; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170984 Copy
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:4879; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170982 Copy
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:41673027
Comments: Please visit https://doi.org/10.1101/2024.07.08.602263 for bioRxiv preprint.
Proper citation: RRID:Addgene_244226 Copy
Species: Synthetic
Genetic Insert: UNS1-P2m-U4d-TurboRFP-t0-UNSX
Vector Backbone Description: Vector Backbone:EML002; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:40998411
Comments: Please visit https://doi.org/10.1101/2025.06.09.658710 for bioRxiv preprint.
Proper citation: RRID:Addgene_238394 Copy
Species: Synthetic
Genetic Insert: UNS1-P2m-U1d-TurboRFP-t0-UNSX
Vector Backbone Description: Vector Backbone:EML002; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:40998411
Comments: Please visit https://doi.org/10.1101/2025.06.09.658710 for bioRxiv preprint.
Proper citation: RRID:Addgene_238392 Copy
Species: Synthetic
Genetic Insert: UNS1-P4m-U3d-TurboRFP-t0-UNSX
Vector Backbone Description: Vector Backbone:EML002; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:40998411
Comments: Please visit https://doi.org/10.1101/2025.06.09.658710 for bioRxiv preprint.
Proper citation: RRID:Addgene_238389 Copy
Species: Synthetic
Genetic Insert: UNS1-P3m-U3d-TurboRFP-t0-UNSX
Vector Backbone Description: Vector Backbone:EML002; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:40998411
Comments: Please visit https://doi.org/10.1101/2025.06.09.658710 for bioRxiv preprint.
Proper citation: RRID:Addgene_238387 Copy
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