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Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90297 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90299 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90253 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24681967
Comments: SAD1 gene with 800 bp upstream of the start site and 900 bp downstream from the stop codon also included
Proper citation: RRID:Addgene_90308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90262 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Comments: Addgene NGS results found F165L and Y390F within the SUB2 translation compared to NP_010199.1
Proper citation: RRID:Addgene_90454 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Proper citation: RRID:Addgene_90456 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7255; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90518 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3831; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90517 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR6
Vector Backbone Description: Vector Backbone:pSE358; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2149118
Proper citation: RRID:Addgene_90468 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100989 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28829039
Proper citation: RRID:Addgene_100991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29207996
Proper citation: RRID:Addgene_101168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPA1
Vector Backbone Description: Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9730822
Comments: RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed)
Proper citation: RRID:Addgene_102614 Copy
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