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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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EcPhnD90.L1∆∆AD.L297RL301R.pRSET Resource Report Resource Website |
RRID:Addgene_33376 | phnD based Phosphonate Biosensor | E. coli | Ampicillin | PMID:22019591 | The EcPhnD-cpGFP insertion variants were constructed by overlap PCR using the synthetic EcPhnD sequence and the cpGFP146 variant from GCaMP2. Detailed sequences are provided in attached sheets. The linker regions were mutated by single-stranded template mutagenesis | Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cpGFP146 variant from GCaMP2 inserted in place of AA89-90 of phnD, L297R in phnD. | 2026-08-29 01:04:08 | 0 |
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EcMBP165-cpGFP.PPYF.pRSET Resource Report Resource Website |
RRID:Addgene_33372 | MBP based Maltose Biosensor | E. coli | Ampicillin | PMID:21989929 | The MBP-cpGFP insertion variant was constructed by overlap PCR using the wild-type MBP sequence from the pMalE-p4E vector (New England Biolabs) and the cpGFP146 variant from GCaMP2 into pRSET-A between BamHI and EcoRI sites. The N-terminal periplasmic leader sequence of MBP was excluded, and a BamHI site (encoding Gly-Ser) was included at the 5′-end. At the C-terminus, an additional His6Gly tag was included after the terminus of MBP (a cloning oversight). An EcoRI site was included after the stop codon. | Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cpGFP146 variant from GCaMP2 inserted between AA165-166 of MBP | 2026-08-29 01:04:08 | 0 |
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pMAL-EFSep Resource Report Resource Website |
RRID:Addgene_34627 | EF-Sep | E. coli | Ampicillin | PMID:21868676 | To construct pMAL-EFSep the gene encoding EF-Sep, was cloned between the NdeI and BamHI sites in the pET20b plasmid (Novaven) to add a C-terminal His6 tag. This fusion construct was then PCR-amplified using primers adding MfeI and PstI restriction sites. The PCR product was cloned in-frame between EcoRI and PstI in pMALc2x (New England Biolabs) to add an N-terminal maltose binding protein (MBP) tag. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid. | Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe219 to Tyr, Thr229 to Ser, Asn274 to Trp, and Val 351 Leu | 2026-08-29 01:04:14 | 0 |
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pNS207 Resource Report Resource Website |
RRID:Addgene_35041 | cytochrome b562 | e. coli | Ampicillin | PMID:1761034 | Vector Backbone:pUC 18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:17 | 0 | ||
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pInducer10-mir-RUP-PheS Resource Report Resource Website 10+ mentions |
RRID:Addgene_44011 | PheS Gly294 | E. coli | Ampicillin | PMID:21307310 | Backbone Marker:Open Biosystems; Backbone Size:13167; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:41 | 19 | ||
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pSO221 Phis-72 BirA GFP Resource Report Resource Website |
RRID:Addgene_45100 | BirA::GFP | E. coli | Ampicillin | PMID:22219512 | Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:49 | 0 | ||
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pBADmod1-linker-deGFP Resource Report Resource Website |
RRID:Addgene_45397 | deGFP | e. coli | Ampicillin | PMID:20576148 | Backbone Marker:Invitrogen; Backbone Size:4102; Vector Backbone:pBAD/His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:51 | 0 | ||
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pBEST-p15A-PLtetO1-deGFP-T500 Resource Report Resource Website |
RRID:Addgene_45392 | deGFP | e. coli | Ampicillin | PMID:20576148 | Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:57 | 0 | ||
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pBEST-p15A-PLtetO1-tetR-T500 Resource Report Resource Website |
RRID:Addgene_45391 | tet repressor | e. coli | Ampicillin | PMID:20576148 | Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:51 | 0 | ||
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pBEST-p15A-PLlacO1-deGFP-T500 Resource Report Resource Website 1+ mentions |
RRID:Addgene_45394 | deGFP | e. coli | Ampicillin | PMID:20576148 | Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:51 | 1 | ||
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pFA-eCA Resource Report Resource Website |
RRID:Addgene_133010 | Can | E. coli | Chloramphenicol | PMID:31406332 | Backbone Size:2970; Vector Backbone:pFA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 12:55:24 | 0 | ||
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pUC-GFP-AT Resource Report Resource Website 1+ mentions |
RRID:Addgene_133306 | axe/txe toxin-antitoxin cassette | E. coli | Kanamycin | PMID:30954530 | Backbone Marker:Oxford Genetics; Backbone Size:4700; Vector Backbone:pSF_UC_OXB20-daGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 12:55:27 | 2 | ||
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pSigma54-4 Resource Report Resource Website |
RRID:Addgene_41748 | rpoN | E. coli | Ampicillin | PMID:22341441 | Backbone Marker:novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | c198a c307g c346a | 2026-08-29 01:05:19 | 0 | |
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pEQ111m Resource Report Resource Website 1+ mentions |
RRID:Addgene_40190 | EcoRI endonuclease & EcoRI methylase | E. coli | Ampicillin | PMID:2745418 | pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | changed Glutamic Acid 111 to Glutamine | 2026-08-29 01:05:03 | 2 |
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SW5 Resource Report Resource Website |
RRID:Addgene_40108 | Bistable switch using dynamic degradation | E. Coli | Kanamycin | PMID:22776405 | Backbone Size:3460; Vector Backbone:psc101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 01:05:03 | 0 | ||
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PRM-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_40127 | CI inducible sfGFP | E. Coli | Ampicillin | PMID:22776405 | Backbone Size:2097; Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-29 01:05:03 | 2 | ||
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PLlacO-1-CImf Resource Report Resource Website |
RRID:Addgene_40126 | Lac inducible lambda repressor | E. Coli | Chloramphenicol | PMID:22776405 | Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:05:03 | 0 | ||
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pAT222 Resource Report Resource Website |
RRID:Addgene_40942 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. Alternate plasmid name: pAT123LacI2G(4mutations) | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | 2026-08-29 01:05:10 | 0 |
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R4-LexAp65-R3 Resource Report Resource Website |
RRID:Addgene_41438 | LexAp65 | E. coli | Kanamycin | Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:05:16 | 0 | |||
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pJB042 (FtsZ-mEos2) Resource Report Resource Website 1+ mentions |
RRID:Addgene_49764 | FtsZ-mEos2 | E. coli | Chloramphenicol | PMID:23859153 | Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:28 | 1 |
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