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http://www.addgene.org/40239

Species: Synechococcus elongatus PCC 7942
Genetic Insert: Cm Resistance, neutral site II integration platform for Synechococcus
Vector Backbone Description: Vector Backbone:1572; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector cloning sites: NheI, XhoI, SmaI, XbaI, StuI, SalI, and EcoRV. Chloramphenicol cassette from pUC1819 (Cm resistant) as a BamHI fragment into BglII site of pAM1572 such that it reads away from the MCS and a ScaI digest gives a 3.9kb band. NSII vector; unique cloning sites NheI, XhoI, SmaI, XbaI, StuI, SalI, EcoRV.

Proper citation: RRID:Addgene_40239 Copy   

•   Source: Addgene

http://www.addgene.org/40241

Species: Synechococcus elongatus PCC 7942
Genetic Insert: promoterless luxAB reporter vector
Vector Backbone Description: Vector Backbone:1573; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector Lux Genes: luxAB cloning sites: NheI, XhoI, SmaI, XbaI, StuI, and SalI. luxAB as SalI-PvuII from pLAVI (pAM884) into EcoRV-SalI cut pAM1573. SalI, StuI, XbaI, SmaI, XhoI, NheI still unique. Glows in E. coli.

Proper citation: RRID:Addgene_40241 Copy   

•   Source: Addgene

http://www.addgene.org/40728

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please note that the hygromycin cassette in this plasmid is non-functional. See provided protocol for high-throughput, ligation-independent cloning using this plasmid.

Proper citation: RRID:Addgene_40728 Copy   

•   Source: Addgene

http://www.addgene.org/40999

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20733051
Comments: Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998). The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended). This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines. For subcloning, the following restriction sites are present: Between the CMV promoter and the Gateway cassette: PmeI (non-unique) Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)

Proper citation: RRID:Addgene_40999 Copy   

•   Source: Addgene

http://www.addgene.org/41439

Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.

Proper citation: RRID:Addgene_41439 Copy   

•   Source: Addgene

http://www.addgene.org/41390

Vector Backbone Description: Backbone Size:9260; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression. Alternate plasmid names: pLX305, SV40-puro; PGK-gateway-no tag

Proper citation: RRID:Addgene_41390 Copy   

•   Source: Addgene

http://www.addgene.org/41393

Vector Backbone Description: Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid expresses rtTA-VP16-2A-puro from the PGK promoter, so it can be used as an 'all-in-one' dox on system. Alternate plasid names: pLIX_401 TRE-gateway; PGK-rtTA-2A-puro

Proper citation: RRID:Addgene_41393 Copy   

•   Source: Addgene

http://www.addgene.org/51481

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.

Proper citation: RRID:Addgene_51481 Copy   

•   Source: Addgene

http://www.addgene.org/51483

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.

Proper citation: RRID:Addgene_51483 Copy   

•   Source: Addgene

http://www.addgene.org/45443

Vector Backbone Description: Backbone Marker:Connie Cepko lab (Harvard Medical School, Boston, MA); Backbone Size:7349; Vector Backbone:pEF-GFP (Addgene Plasmid #11154); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18754012
Comments: Alternate plasmid name: pEF1α-RfC.1-IRES-GFP To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP. Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.

Proper citation: RRID:Addgene_45443 Copy   

•   Source: Addgene

http://www.addgene.org/45957

Vector Backbone Description: Backbone Size:9500; Vector Backbone:pCWX-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21590398
Comments: More information can be found at http://lentilab.unige.ch/ .

Proper citation: RRID:Addgene_45957 Copy   

•   Source: Addgene

http://www.addgene.org/45952

Vector Backbone Description: Backbone Size:10921; Vector Backbone:pCLX-pTF-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21761975
Comments: More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html

Proper citation: RRID:Addgene_45952 Copy   

•   Source: Addgene

http://www.addgene.org/46339

Vector Backbone Description: Backbone Marker:gift of W. Schaffner, University of Zurich; Backbone Size:6922; Vector Backbone:pSCT-GAL33VP80; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22966205

Proper citation: RRID:Addgene_46339 Copy   

•   Source: Addgene

http://www.addgene.org/46646

Genetic Insert: mir-1-1 reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression, RNAi, Expression of query pri-miRNA sequences in HEK293 cells; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23415231
Comments: Query pri-miRNA sequences are to be recombined into this plasmid using Gateway recombination. The human pri-mir-1-1 sequence is cloned downstream of the query pri-miRNA and is transcriptionally fused to the query pri-miRNA sequence. This plasmid was used for ectopic pri-miRNA expression in HEK293 cells

Proper citation: RRID:Addgene_46646 Copy   

•   Source: Addgene

http://www.addgene.org/47032

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006 After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.

Proper citation: RRID:Addgene_47032 Copy   

•   Source: Addgene

http://www.addgene.org/47074

Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_47074 Copy   

•   Source: Addgene

http://www.addgene.org/47072

Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_47072 Copy   

•   Source: Addgene

http://www.addgene.org/47078

Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_47078 Copy   

•   Source: Addgene

http://www.addgene.org/47077

Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:6247; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_47077 Copy   

•   Source: Addgene

http://www.addgene.org/47069

Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5824; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291

Proper citation: RRID:Addgene_47069 Copy   

•   Source: Addgene