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Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65609 Copy
Species: E. coli
Genetic Insert: FadD with Q338R mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65567 Copy
Species: E. coli
Genetic Insert: FadD with V4F, W5L mutations
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65570 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65575 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65578 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65577 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65610 Copy
Species: E. coli
Genetic Insert: FadD with V451A mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65572 Copy
Species: E. coli
Genetic Insert: FadD with F447S mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65571 Copy
Species: E. coli
Genetic Insert: FadD D372G
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65573 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65580 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65587 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65586 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65588 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65583 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65584 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65590 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65598 Copy
Species: E. coli
Genetic Insert: ssb (W88C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20350930
Comments: Please note- The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., W89C = W88C).
The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine. The adduct with Cy3B maleimide has been used as a biosensor for single-stranded DNA in TIRF microscopy measurements.
Proper citation: RRID:Addgene_78204 Copy
Species: E. coli
Genetic Insert: ssb (G26C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599625
Comments: Please note: The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., G27C = G26C).
The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~6-fold fluorescence increase on binding ssDNA with very high affinity.
Proper citation: RRID:Addgene_78203 Copy
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