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Species: bacteriophage MS2
Genetic Insert: NC-MCP (MS2 coat protein) fusion protein in Gag
Vector Backbone Description: Vector Backbone:psPAX2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759231
Comments: Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_122944 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9-PP7-HBB 3' UTR
Vector Backbone Description: Vector Backbone:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759231
Proper citation: RRID:Addgene_122947 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9-MS2-HBB 3' UTR
Vector Backbone Description: Vector Backbone:pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759231
Proper citation: RRID:Addgene_122946 Copy
Genetic Insert: CfaN
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26854538
Comments: Note: Plasmid contains a 5xHis tag rather than 6xHis tag.
Proper citation: RRID:Addgene_122949 Copy
Species: Homo sapiens
Genetic Insert: EGFP-alpha Tubulin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11294900
Comments: EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech
Proper citation: RRID:Addgene_12298 Copy
Species: Homo sapiens
Genetic Insert: MyD88
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Addgene's sequencing results indicate the MyD88 insert starts at aa15 when compared to GenBank reference sequence NP_002459.2 (MyD88 isoform 2). When numbered according to this reference sequence, the MyD88 mutation is I192N.
Proper citation: RRID:Addgene_12295 Copy
Species: Homo sapiens
Genetic Insert: alpha Tubulin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14588246
Comments: PA-GFP-Tubulin came from the pPA-GFP-alpha Tubulin vector
Proper citation: RRID:Addgene_12297 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12293 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12292 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YML029W (Marked)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4365; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS306SpeI-UP YML029W-TFs-[tetO2]2-TATA_YML029W_4silmut_NMD2intron_RS_BgllI-NotI
Proper citation: RRID:Addgene_122940 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBR026C (Marked)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4807; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS306KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YBR026C_4silmut_RS_BamHI-EcoRI-Ter-NotI
Proper citation: RRID:Addgene_122934 Copy
Species: Homo sapiens
Genetic Insert: STAT1 Y701F
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. Y701F mutation made using QuikChange mutagenesis kit. The STAT1 insert contains a N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YML043C (Marked)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4805; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS306KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YML043C_4silmut_RS_MfeI-EcoRI-Ter-NotI
Proper citation: RRID:Addgene_122936 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YML070W (Marked)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4807; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS306KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YML070W_4silmut_RS_BamHI-EcoRI-Ter-NotI
Proper citation: RRID:Addgene_122938 Copy
Species: Homo sapiens
Genetic Insert: STAT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. The hSTAT1 insert contains an N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12301 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YML071C (Marked)
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4805; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS306-KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YML071C_4silmut_RS_MfeI-EcoRI-Ter-NotI
Proper citation: RRID:Addgene_122939 Copy
Genetic Insert: mTRF2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2960; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9326950
Proper citation: RRID:Addgene_12300 Copy
Species: Homo sapiens
Genetic Insert: RALA
Vector Backbone Description: Vector Backbone:pET302/NT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30500825
Proper citation: RRID:Addgene_122930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tTA
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5641; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28706991
Comments: pRS305-EagI-Pcln3-BamHI-tTA-EcoRI-Tcyc1-ApaI. Plasmid contains rTetR, a part of the tet system. Origin of the tet system: Gossen and Bujard, 1992.
Proper citation: RRID:Addgene_122932 Copy
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