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Species: Synthetic
Genetic Insert: p35S:MS2-GFP:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-GFP coding sequence = 1542-2846bp
RBCS terminator = 2851-3496bp
BsaI site #1 = 3531-3536bp
BsaI site #2 = 3545-3550bp
Proper citation: RRID:Addgene_122859 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)_mouseL_ACP
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122850 Copy
Species: streptococcus pyogenes
Genetic Insert: Cas9-GFP
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2700; Vector Backbone:pUC19; Vector Types:CRISPR, Cas9-GFP plasmid for genome editing of Cryptosporidium parasites; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31231046
Proper citation: RRID:Addgene_122853 Copy
Species: streptococcus pyogenes
Genetic Insert: Cas9-GFP
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2700; Vector Backbone:pUC19; Vector Types:CRISPR, Cas9-GFP plasmid for genome editing of Cryptosporidium parasites; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31231046
Proper citation: RRID:Addgene_122852 Copy
Species: Arabidopsis thaliana
Genetic Insert: SET catalytic domain of KYP H3K9 histone methyltransferase
Vector Backbone Description: Backbone Size:2688; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
KYP coding sequence = 2254-3060bp
BsaI site #2 = 3066-3071bp
Proper citation: RRID:Addgene_122855 Copy
Species: Homo sapiens
Genetic Insert: human FASN/ human fatty acid synthase
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122846 Copy
Vector Backbone Description: Backbone Size:7763; Vector Backbone:pDEST-CMV-C-EGFP; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_122845 Copy
Genetic Insert: Barcode AX
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122726 Copy
Species: Mus musculus
Genetic Insert: mouse FASN/ mouse fatty acid synthase
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122847 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122849 Copy
Species: Synthetic
Genetic Insert: SRDX transcriptional repressor domain
Vector Backbone Description: Backbone Size:2691; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
SRDX coding sequence = 2254-2295bp
BsaI site #2 = 2304-2309bp
Proper citation: RRID:Addgene_122840 Copy
Genetic Insert: Barcode AS
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122721 Copy
Species: Synthetic
Genetic Insert: Core domain of p300 H3K27 histone acetyltransferase, codon-optimised for Arabidopsis
Vector Backbone Description: Backbone Size:2743; Vector Backbone:pUC57; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 445-450bp
p300 coding sequence = 458-2311bp
BsaI site #2 = 2320-2325bp
Proper citation: RRID:Addgene_122841 Copy
Vector Backbone Description: Backbone Size:7720; Vector Backbone:pDEST-CMV-N-EGFP; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_122843 Copy
Species: Homo sapiens
Genetic Insert: NOTCH2NL-delta C terminus
Vector Backbone Description: Backbone Marker:Dr. Cecile Charrier; Vector Backbone:lentiviral; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29856955
Proper citation: RRID:Addgene_122956 Copy
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123001 Copy
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123003 Copy
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123005 Copy
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123009 Copy
Vector Backbone Description: Backbone Marker:Dr. Cecile Charrier; Vector Backbone:lentiviral; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29856955
Proper citation: RRID:Addgene_122953 Copy
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