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Genetic Insert: TDK gene promoter and dCas9
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Note: This plasmid contains a L64H mutation in Cas9. This mutation is not known to affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113657 Copy
Genetic Insert: mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113750 Copy
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2-mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113753 Copy
Genetic Insert: mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113752 Copy
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113751 Copy
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3048; Vector Backbone:pGEM-T easy; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
Proper citation: RRID:Addgene_113758 Copy
Genetic Insert: mRuby2-mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113757 Copy
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113756 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has aminoglycosyl phosphotransferase driven by 35S promoter (G418 resistance). We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113743 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has hygromycinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113742 Copy
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR221-P5P4; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113741 Copy
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113746 Copy
Species: Synthetic
Genetic Insert: stop cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to generate a premature stop codon at your locus of interest. Also contains a multiple cloning site for easy genotyping. This plasmid contains multiple M13F and M13R sites, due to cloning the MCS of pBluescriptSK+.
QC note: This plasmid dimerizes during growth in bacteria, which can result in digest and Sanger sequencing results that differ from the full plasmid sequence of the monomer. The depositing lab has confirmed that dimerization does not affect plasmid function.
Proper citation: RRID:Addgene_113745 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has zeocinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113744 Copy
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP-mEGFP-mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113748 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113691 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113695 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113694 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113693 Copy
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:AAV; Vector Types:Mouse Targeting, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: CREB gRNA sequence: GGAGCAGACAACCAGCAGAG
Proper citation: RRID:Addgene_113692 Copy
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