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Species: Homo sapiens
Genetic Insert: irisin (ectodomain of FNDC5)
Vector Backbone Description: Backbone Marker:novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25749243
Proper citation: RRID:Addgene_122612 Copy
Genetic Insert: evoCDA1-BE4max
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-ks101DXbKJKwRUhkSwYB for additional information.
Proper citation: RRID:Addgene_122608 Copy
Genetic Insert: CDA1-BE4max
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-zbEZMpRhD1Jq00X1ntJn for additional information.
Proper citation: RRID:Addgene_122607 Copy
Species: Mus musculus
Genetic Insert: Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122681 Copy
Genetic Insert: Barcode AL
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122714 Copy
Species: Arabidopsis thaliana
Genetic Insert: SUC2 promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
SUC2 promoter = 2252-4381bp
BsaI site #2 = 4387-4392bp
Proper citation: RRID:Addgene_122835 Copy
Species: Synthetic
Genetic Insert: MS2-NLS, codon-optimised for Arabidopsis
Vector Backbone Description: Backbone Size:2149; Vector Backbone:pMK-RQ; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 380-385p
MS2-NLS coding sequence = 393-872bp
BsaI site #2 = 878-883bp
Proper citation: RRID:Addgene_122837 Copy
Genetic Insert: Barcode AM
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122715 Copy
Genetic Insert: Barcode AP
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122718 Copy
Species: Synthetic
Genetic Insert: VP64 transcriptional activator domain
Vector Backbone Description: Backbone Size:2404; Vector Backbone:pMA-RQ; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
VP64 coding sequence = 2254-2421bp
BsaI site #2 = 2430-2435bp
Proper citation: RRID:Addgene_122839 Copy
Species: Homo sapiens
Genetic Insert: MyD88
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Addgene's sequencing results indicate the MyD88 insert starts at aa15 when compared to GenBank reference sequence NP_002459.2 (MyD88 isoform 2). When numbered according to this reference sequence, the MyD88 mutation is P213H
Proper citation: RRID:Addgene_12288 Copy
Genetic Insert: Barcode AO
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122717 Copy
Species: Pisum sativum (pea)
Genetic Insert: RBCS terminator
Vector Backbone Description: Backbone Size:2687; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
RBCS terminator = 2252-2897bp
BsaI site #2 = 2904-2909bp
Proper citation: RRID:Addgene_122838 Copy
Species: Homo sapiens
Genetic Insert: SHP-2CS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3500; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15133037
Proper citation: RRID:Addgene_12284 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations A74V, M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12290 Copy
Vector Backbone Description: Vector Backbone:ICE plasmid; Vector Types:Yeast Expression, Yeast Recombination Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31074531
Proper citation: RRID:Addgene_122890 Copy
Vector Backbone Description: Vector Backbone:ICE plasmid; Vector Types:Yeast Expression, Yeast Recombination Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31074531
Proper citation: RRID:Addgene_122893 Copy
Vector Backbone Description: Vector Backbone:ICE plasmid; Vector Types:Yeast Expression, Yeast Recombination Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31074531
Proper citation: RRID:Addgene_122895 Copy
Vector Backbone Description: Vector Backbone:ICE plasmid; Vector Types:Yeast Expression, Yeast Recombination Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31074531
Proper citation: RRID:Addgene_122896 Copy
Vector Backbone Description: Vector Backbone:ICE plasmid; Vector Types:Yeast Expression, Yeast Recombination Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31074531
Proper citation: RRID:Addgene_122899 Copy
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