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pVF30
 
Resource Report
Resource Website 		RRID:Addgene_229472 RssB (R115A) E. coli Ampicillin PMID:30975721 Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin R115A 2026-08-15 01:31:36 0
G555
 
Resource Report
Resource Website 		RRID:Addgene_216483 plsB, plsC, cdsA, pssA E. coli Ampicillin PMID:40471122 Please visit https://doi.org/10.1101/2024.03.22.586135 for bioRxiv preprint. Backbone Size:3162; Vector Backbone:pUC57-oriL191-oriR194; Vector Types:Cell-free expression; Bacterial Resistance:Ampicillin 2026-08-15 01:29:28 0
DHIP1
 
Resource Report
Resource Website 		RRID:Addgene_226851 DH10β atpi:slr0214:gidB E. coli Streptomycin PMID:39846505 Strain has resistance to streptomycin but not spectinomycin, use spectinomycin 100 µg/mL for plasmids with a streptomycin resistance cassette. This strain methylates the first cytosine in the palindrome CGATCG. Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin 2026-08-15 01:31:17 0
pLVX-TetOne-Puro-mitoEcSTH-FLAG
 
Resource Report
Resource Website 		RRID:Addgene_218629 mitoEcSTH E. coli Ampicillin PMID:37884806 Backbone Marker:Clontech; Backbone Size:9227; Vector Backbone:pLVX-TetOne-Puro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:29:58 0
ATMY1
 
Resource Report
Resource Website 		RRID:Addgene_218770 EcNR1GT pUltraBR MjY dtyrS dtyrTV::tolC tyrU::gentR lambda::ZeoR E. coli Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL) PMID:30078635 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL) 2026-08-15 01:30:01 0
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
 
Resource Report
Resource Website 		RRID:Addgene_218766 EcTyrRS-VSMA* E. coli Chloramphenicol PMID:38913984 EcY-tRNA-TAG After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome. Backbone Size:4091; Vector Backbone:pEVOL-tac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Y37V, D167G, D182S, F183M, L186A, D265R 2026-08-15 01:29:59 0
pEvoltac EcW-TGA-h9
 
Resource Report
Resource Website 		RRID:Addgene_218765 EcWRS-h9 E. coli Chloramphenicol PMID:28192410 EcW-tRNA-TGA After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome. Backbone Size:4166; Vector Backbone:pEvoltac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol S8A, V144S, V146A 2026-08-15 01:29:59 0
ATMY5
 
Resource Report
Resource Website 		RRID:Addgene_218773 EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolC E. coli Spectinomycin PMID:30078635 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:30:01 0
ATMY-C321
 
Resource Report
Resource Website 		RRID:Addgene_218774 C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR E. coli Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL) PMID:38913984 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL) 2026-08-15 01:29:59 0
ATMY3
 
Resource Report
Resource Website 		RRID:Addgene_218771 EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolC E. coli Spectinomycin PMID:30078635 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:59 0
ATMY4
 
Resource Report
Resource Website 		RRID:Addgene_218772 EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolC E. coli Spectinomycin PMID:30078635 Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:59 0
pET28a_speG_Cterm_His
 
Resource Report
Resource Website 		RRID:Addgene_220483 speG E. coli Kanamycin PMID:38885650 Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin none 2026-08-15 01:30:06 0
pNP568
 
Resource Report
Resource Website 		RRID:Addgene_222725 IS621 bridge RNA E. coli Kanamycin PMID:38926615 Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint. Vector Backbone:pNP555 - ColE1 Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:30:13 0
pNP2270
 
Resource Report
Resource Website 		RRID:Addgene_222733 IS621 Helper (lacZ) E. coli Chloramphenicol PMID:38926615 Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint. Vector Backbone:pNP1950 - p15A Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:30:13 0
PT7lacO-His6-ThrRS
 
Resource Report
Resource Website 		RRID:Addgene_224365 ThrRS E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:24 0
PT7lacO-His6-AlaRS
 
Resource Report
Resource Website 		RRID:Addgene_224362 AlaRS E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:24 0
PT7lacO-EF-G-His8
 
Resource Report
Resource Website 		RRID:Addgene_224369 EF-G E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
PT7lacO-His6-IF3
 
Resource Report
Resource Website 		RRID:Addgene_224368 IF3 E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
PT7lacO-RF3-His6
 
Resource Report
Resource Website 		RRID:Addgene_224373 RF3 E. coli Ampicillin PMID:40209036 It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint. Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:30:25 0
pGLU_67
 
Resource Report
Resource Website 		RRID:Addgene_225770 Paph(3')-I_[abr] E. Coli Chloramphenicol Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol N/A 2026-08-15 01:30:44 0

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