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Species: E. coli
Genetic Insert: DNA polymerase I
Vector Backbone Description: Backbone Marker:see NCBI D88215; Backbone Size:3475; Vector Backbone:pHSG576; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:12909725
Comments: Control for random mutagenesis experiments. See attached mutagenesis protocol provided by depositing scientist.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_11721 Copy
Species: E. coli
Genetic Insert: DNA polymerase I EP
Vector Backbone Description: Backbone Marker:see NCBI D88215; Backbone Size:3475; Vector Backbone:pHSG576; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:12909725
Comments: See attached mutagenesis protocol provided by depositing scientist.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_11722 Copy
Species: E. coli
Genetic Insert: cda-GFPuv
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1788; Vector Backbone:pSMART; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_102283 Copy
Species: E. coli
Genetic Insert: pfkB gRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_102284 Copy
Species: E. coli
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen/EMD Millipore; Backbone Size:5227; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27795317
Comments: This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene.
Proper citation: RRID:Addgene_103064 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR-Gly9-DDX6 (contains SapI)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115949 Copy
Species: E. coli
Genetic Insert: E. coli gRNAs
Vector Backbone Description: Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_115927 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105848 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105846 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105847 Copy
Species: E. coli
Genetic Insert: araE
Vector Backbone Description: Vector Backbone:pSEVA221; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29299733
Proper citation: RRID:Addgene_110141 Copy
Species: E. coli
Genetic Insert: Modified PrhaBAD (CRP-binding sites removed)
Vector Backbone Description: Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29544054
Proper citation: RRID:Addgene_110545 Copy
Species: E. coli
Genetic Insert: PrhaBAD
Vector Backbone Description: Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29544054
Proper citation: RRID:Addgene_110544 Copy
Species: E. coli
Genetic Insert: 2HMG-mCherry
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_112739 Copy
Species: E. coli
Genetic Insert: 2(KW)2-eGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26264666
Proper citation: RRID:Addgene_112736 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pHMGWA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113127 Copy
Species: E. coli
Genetic Insert: IHFa E10A
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113243 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113242 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113241 Copy
Species: E. coli
Genetic Insert: IHFa K20A
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113247 Copy
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