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Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41611 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC63-mRFP
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18812321
Comments: Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function.
Proper citation: RRID:Addgene_41842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AVT1
Vector Backbone Description: Backbone Marker:Dan Gottschling (Addgene plasmid# 41894); Backbone Size:7882; Vector Backbone:pAG306-GPD-ccdB chr I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172144
Proper citation: RRID:Addgene_41898 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mediator of the Replication Checkpoint
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6021; Vector Backbone:pRS415; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12865299
Comments: There is a D304N mutation in LEU, relative to aa1 in LEU, it is not thought to affect function.
Proper citation: RRID:Addgene_41924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Backbone Marker:ATCC, Number: 77142; Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23222782
Proper citation: RRID:Addgene_40276 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERV1
Vector Backbone Description: Vector Backbone:pDisCoTune; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34405535
Proper citation: RRID:Addgene_173713 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Please note: Plasmid contains E10G and N257D mutations in URA3. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_174064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-LacO-3NLS-CIB1-VP16-tADH1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Please note: The plasmid contains a 217bp insertion between Tadh1 and HIS3 3'UTR compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_174066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEF1-3NLS-97-3ZF-VP16
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174068 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174063 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GALL promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2574; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17418 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2581; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:2691; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64536; Backbone Size:4434; Vector Backbone:pRG206MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154821 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway middle entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32697972
Comments: Can be recombined with any p5E vector containing a promoter to allow for tissue-specific expression of QFGal4. Note that an SV40pA is included after QFGal4.
Proper citation: RRID:Addgene_155118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4
Vector Backbone Description: Backbone Size:4053; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Expression in zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697972
Comments: Can be linearized with NotI for in vitro synthesis of QFGal4
Proper citation: RRID:Addgene_155126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P2A-QFGal4
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:3310; Vector Backbone:pK; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32697972
Comments: pGTag series vectors were described in PMID: 32412410. pGTag-P2A-QFGal4-SV40 was modified from pGTag-eGFP-SV40 (Plasmid #117813).
Proper citation: RRID:Addgene_155127 Copy
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