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Species: Oryza sativa (rice)
Genetic Insert: ORPHANS transcription factor encoded by Os02g0646200 LOC_Os02g43170
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27901 Copy
Species: Zea mays
Genetic Insert: DBP transcription factor from EB701836
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27865 Copy
Species: Zea mays
Genetic Insert: HB homeobox transcription factor from EE011258
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27866 Copy
Species: Zea mays
Genetic Insert: FAR1 transcription factor from DR827144
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27864 Copy
Species: Oryza sativa (rice)
Genetic Insert: WRKY transcription factor encoded by Os05g0183100 LOC_Os05g09020
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27957 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os03g0759700 LOC_Os03g55220
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27958 Copy
Species: Oryza sativa (rice)
Genetic Insert: WRKY transcription factor encoded by Os01g0714800 LOC_Os01g51690
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27955 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os03g0741100 LOC_Os03g53020
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27953 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os08g0524800 LOC_Os08g41320
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27951 Copy
Species: Xanthomonas
Genetic Insert: TAL-monomer-HD
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27968 Copy
Species: Synthetic
Genetic Insert: TALE1
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27969 Copy
Species: Xanthomonas
Genetic Insert: TAL-monomer-NI
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27966 Copy
Species: Xanthomonas
Genetic Insert: TAL-monomer-NG
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27967 Copy
Species: Xanthomonas
Genetic Insert: TAL-monomer-NN
Vector Backbone Description: Backbone Size:2672; Vector Backbone:pJ201; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27965 Copy
Species: Synthetic
Genetic Insert: TAL-Backbone(NI)
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_27962 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os01g0928000 LOC_Os01g70310
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27960 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting NFATc1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NFATc1(see below). Please note that this plasmid does NOT contain the NFATc1 sequence.
Users must order the complementary plasmid NFATc1_L (OZ543) [Addgene plasmid 28068] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGGA-3' in a bacterial two hybrid assay, and resulted in 7.97 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28069 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting Encephalopsin (Opn3)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence.
Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28066 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting Atg7
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Atg7 (see below). Please note that this plasmid does NOT contain the Atg7 sequence.
Users must order the complementary plasmid Atg7_L (OZ557) [Addgene plasmid 28082] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GTGGTGGCT-3' in a bacterial two hybrid assay, and resulted in 6.91 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28083 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting cdkn1a(p21)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene cdkn1a(p21) (see below). Please note that this plasmid does NOT contain the cdkn1a(p21) sequence.
Users must order the complementary plasmid cdkn1a(p21)_R (OZ556) [Addgene plasmid 28081] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAGTGAACG-3' in a bacterial two hybrid assay, and resulted in 14.32 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28080 Copy
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