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Genetic Insert: SARS-CoV-2 nucleocapsid
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_158079 Copy
Genetic Insert: SARS-CoV-2 matrix
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_158078 Copy
Species: Homo sapiens
Genetic Insert: miniTurbo-V5-CDK7
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_158194 Copy
Species: Synthetic
Genetic Insert: CLYBL-CAG-DDdCas9VPH-T2A-GFP
Vector Backbone Description: Backbone Size:6069; Vector Backbone:pUCM-CLYBL-hNIL; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34031600
Proper citation: RRID:Addgene_158091 Copy
Species: Other
Genetic Insert: SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32641830
Proper citation: RRID:Addgene_158092 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme UbE2U
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4900; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17597759
Proper citation: RRID:Addgene_15810 Copy
Genetic Insert: mTagBFP
Vector Backbone Description: Backbone Size:6494; Vector Backbone:pCTCON2-RXG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158127 Copy
Genetic Insert: mTagBFP
Vector Backbone Description: Backbone Size:6494; Vector Backbone:pCTCON2-RYG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158126 Copy
Species: Homo sapiens
Genetic Insert: Cullin2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16338364
Comments: C-terminal truncation of hCul2: amino acids 1-427.
Proper citation: RRID:Addgene_15819 Copy
Species: Rattus norvegicus
Genetic Insert: Rat ACE2
Vector Backbone Description: Vector Backbone:pscALPS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_158086 Copy
Species: Homo sapiens
Genetic Insert: TSP2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: 2685bp insert of hTSP2 promoter includes exon 1A and exon 1B.
Proper citation: RRID:Addgene_15816 Copy
Genetic Insert: BFP-GFP fragment
Vector Backbone Description: Backbone Size:4693; Vector Backbone:pCTCON2-BYG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158137 Copy
Genetic Insert: BFP-GFP fragment
Vector Backbone Description: Backbone Size:4693; Vector Backbone:pCTCON2-BXG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158132 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pCTCON2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158130 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pCTCON2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158131 Copy
Species: Other
Genetic Insert: COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32641830
Proper citation: RRID:Addgene_158095 Copy
Genetic Insert: Aga1p-FAPB2.3.6
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158147 Copy
Genetic Insert: Aga1p-FAPB2.3.6
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158148 Copy
Genetic Insert: BFP-GFP fragment (w/ TAG codon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158145 Copy
Genetic Insert: BFP-GFP fragment (w/ TAG codon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33791108
Proper citation: RRID:Addgene_158146 Copy
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