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Species: Homo sapiens
Genetic Insert: TDP43
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30500534
Comments: Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.
Proper citation: RRID:Addgene_122669 Copy
Vector Backbone Description: Backbone Size:4703; Vector Backbone:This is a new backbone created by fusing the FRT-flanked kanamycin resistance gene of pKD13 into the MCS of pPCR Script Cam SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122702 Copy
Genetic Insert: Barcode AB
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122704 Copy
Genetic Insert: Barcode AE
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122707 Copy
Genetic Insert: Barcode AG
Vector Backbone Description: Backbone Marker:Shaw and Bourret; Backbone Size:4703; Vector Backbone:pSKAP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31132072
Proper citation: RRID:Addgene_122709 Copy
Species: Homo sapiens
Genetic Insert: tUI
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122661 Copy
Species: Homo sapiens
Genetic Insert: tISR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122664 Copy
Species: Homo sapiens
Genetic Insert: tIVR
Vector Backbone Description: Backbone Size:5293; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31308549
Proper citation: RRID:Addgene_122663 Copy
Vector Backbone Description: Backbone Size:11086; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.
For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid).
Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.
Packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12262 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:12168; Vector Backbone:pWPT; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter
The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre.
Unique restriction sites at key positions will allow you to change the promoter and transgene.
Please note that ClaI in these vectors is blocked by Dam methylation.
The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning.
Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12261 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:8985; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11020357
Comments: Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12264 Copy
Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_122652 Copy
Species: Listeria innocua
Genetic Insert: DPS, listeria
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14514305
Comments: This plasmid is also used in the following publications:
Resnick, D. A., Gilmore, K., Idzerda, Y. U., Klem, M. T., Allen, M., Douglas, T., … Young, M. (2006). Magnetic properties of Co 3O 4 nanoparticles mineralized in Listeria innocua Dps. Journal of Applied Physics, 99(8), 1–4. https://doi.org/10.1063/1.2163839
Okuda, M., Suzumoto, Y., Iwahori, K., Kang, S., Uchida, M., Douglas, T., & Yamashita, I. (2010). Bio-templated CdSe nanoparticle synthesis in a cage shaped protein, Listeria-Dps, and their two dimensional ordered array self-assembly. Chemical Communications, 46(46), 8797–8799. https://doi.org/10.1039/c0cc03298k
Usselman, R. J., Russek, S. E., Klem, M. T., Allen, M. A., Douglas, T., Young, M., … Singel, D. J. (2012). Temperature dependence of electron magnetic resonance spectra of iron oxide nanoparticles mineralized in Listeria innocua protein cages. Journal of Applied Physics, 112(8), 1–9. https://doi.org/10.1063/1.4757964
Proper citation: RRID:Addgene_122654 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122647 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens forkhead box P2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30887622
Proper citation: RRID:Addgene_122649 Copy
Species: Homo sapiens
Genetic Insert: CXCR4 siRNA
Vector Backbone Description: Backbone Size:7139; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15882617
Comments: CXCR4-siRNA-1 sequence: 5'-TGGAGGGGATCAGTATATACA-3'
Proper citation: RRID:Addgene_12271 Copy
Vector Backbone Description: Backbone Marker:Jan-Willem Veening; Backbone Size:4314; Vector Backbone:pPEPZ-Plac; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31121970
Proper citation: RRID:Addgene_122637 Copy
Species: synthetic, based on superfolder GFP
Genetic Insert: phIorine2
Vector Backbone Description: Backbone Marker:SEVA; Backbone Size:5300; Vector Backbone:pSEVA2513; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31162835
Proper citation: RRID:Addgene_122590 Copy
Species: Pseudomonas putida
Genetic Insert: xylS
Vector Backbone Description: Backbone Size:3147; Vector Backbone:pTn7-M; Vector Types:Bacterial Expression, Synthetic Biology, Tn7 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30864281
Proper citation: RRID:Addgene_122591 Copy
Genetic Insert: Pm promoter
Vector Backbone Description: Backbone Marker:SEVA; Backbone Size:4170; Vector Backbone:pSEVA247M; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30864281
Proper citation: RRID:Addgene_122594 Copy
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