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Species: Homo sapiens
Genetic Insert: Dsh2
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:5500; Vector Backbone:pIRESpuro-GLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547521
Proper citation: RRID:Addgene_15101 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.
Proper citation: RRID:Addgene_15067 Copy
Species: Xenopus laevis
Genetic Insert: BMP2-Vg1
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8348610
Comments: PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.).
See article for more cloning details.
The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.
Proper citation: RRID:Addgene_15071 Copy
Species: Mus musculus
Genetic Insert: p27
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11404411
Proper citation: RRID:Addgene_15191 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725307.1
Proper citation: RRID:Addgene_152009 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725303.1
Proper citation: RRID:Addgene_152008 Copy
Species: Human coronavirus NL63
Genetic Insert: Human coronavirus NL63 nucleocapsid protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: Q6Q1R8
Proper citation: RRID:Addgene_151957 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724396.1
Proper citation: RRID:Addgene_152004 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725312.1
Proper citation: RRID:Addgene_152003 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725297.1
Proper citation: RRID:Addgene_152002 Copy
Species: Mus musculus
Genetic Insert: PIAS3
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMV-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9388184
Comments: Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.
Proper citation: RRID:Addgene_15207 Copy
Species: Mus musculus
Genetic Insert: Chx10 3kb AP reporter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15893976
Comments: The 3Kb-AP reporter was constructed using a
genomic BamHI–SacII fragment isolated from a Chx10
BAC (Rowan and Cepko, 2004) and subcloned into pBluescript
(Stratagene). An SV40 intervening sequence (IVS)
was isolated from pNASSbeta (Clontech) digested with EcoRI
and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was
isolated from the SV40all plasmid (gift of S. Dymecki).
Proper citation: RRID:Addgene_15205 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725307.1
Proper citation: RRID:Addgene_152019 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (Nucleocapsid)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: QHN73817.1
Proper citation: RRID:Addgene_151966 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724395.1
Proper citation: RRID:Addgene_152012 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3'-to-5' exonuclease
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725309.1
Proper citation: RRID:Addgene_152013 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725303.1
Proper citation: RRID:Addgene_151976 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725307.1
Proper citation: RRID:Addgene_152028 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725298.1
Proper citation: RRID:Addgene_151977 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724394.1
Proper citation: RRID:Addgene_152023 Copy
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