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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJL2-mGold-P2A-EBFP2
 
Resource Report
Resource Website
RRID:Addgene_157998 mGold-P2A-EBFP2 Synthetic Ampicillin PMID:33097540 A mammalian expression cassette was cloned into a yeast vector. mGold: FPBaseID TBD EBFP2: FPBaseID DVMQ7 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. Backbone Size:6992; Vector Backbone:pJL2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin mGold is mVenus with L46F;T63S mutations 2026-09-19 02:11:46 0
pJL-mGold
 
Resource Report
Resource Website
RRID:Addgene_157997 mGold Synthetic Ampicillin PMID:33097540 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. Backbone Size:6950; Vector Backbone:pJL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin mGold is mVenus with L46F;T63S mutations 2026-09-19 02:11:46 0
pSFFV_CloGFP(1-10)
 
Resource Report
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RRID:Addgene_157994 CloGFP(1-10) Synthetic Ampicillin PMID:33227014 Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin G32Q, F46L, K101R, S202L 2026-09-19 02:11:46 0
pSFFV_mNG3K(1-10)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_157993 pSFFV_mNG3K(1-10) Synthetic Ampicillin PMID:33227014 Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin G27D, D42E, M128I, K153M, T170I 2026-09-19 02:11:46 2
pCaggs-mGold
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_157996 mGold Synthetic Ampicillin PMID:33097540 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. Backbone Size:6105; Vector Backbone:pCaggs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin mGold is mVenus with L46F;T63S mutations 2026-09-19 02:11:46 1
pVL1393-MAL-SYK
 
Resource Report
Resource Website
RRID:Addgene_157990 Spleen tyrosine kinase Homo sapiens Ampicillin PMID:29890824 Vector Backbone:pVL1393; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:11:46 0
pLKO-CreERT2 sgAdam10 v3
 
Resource Report
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RRID:Addgene_158047 Adam10 sgRNA Mus musculus Ampicillin PMID:32165588 Vector Backbone:pLKO-ERT2 Cre stuffer v3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:11:47 0
pBS-GMR-eya(shRNA)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_157991 Ampicillin PMID:33016195 Backbone Size:2961; Vector Backbone:pBSII-KS(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-09-19 02:11:46 3
pDOC-K-glmS-GFPrev
 
Resource Report
Resource Website
RRID:Addgene_158060 Green Fluorescent Protein optimised for excitation with UV light Synthetic Ampicillin PMID:33226934 Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-19 02:11:47 0
pDOC-K-glmS-lacIZ
 
Resource Report
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RRID:Addgene_158061 lacIZ operon Escherichia coli Ampicillin PMID:33226934 Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-19 02:11:47 0
pDOC-K-glmS
 
Resource Report
Resource Website
RRID:Addgene_158058 glmS homologous region 1 Salmonella enterica Ser. Typhimurium Ampicillin PMID:33226934 Backbone Size:7233; Vector Backbone:pDOC-K; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-19 02:11:47 0
p667-UBC-PHKG1-V5-miniTurbo_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_158192 PHKG1-V5-miniTurbo Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-19 02:11:49 0
p667-UBC-DYRK3-V5-miniTurbo_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_158190 DYRK3-V5-miniTurbo Homo sapiens Ampicillin Depositor confirms R293H does not affect plasmid function. This is a known sequence conflict. These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin R293H- please see depositor comments 2026-09-19 02:11:49 0
pJWK_VD_02
 
Resource Report
Resource Website
RRID:Addgene_158103 5'UTR synthetic stem-loop -30kcal/mol Synthetic Ampicillin PMID:32846140 Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-19 02:11:48 0
pJWK_VD_03
 
Resource Report
Resource Website
RRID:Addgene_158104 5'UTR of CACNA1C (uc009zdu.1) Homo sapiens Ampicillin PMID:32846140 Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-19 02:11:48 0
p667-UBC-BCKDK-V5-miniTurbo_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_158189 BCKDK-V5-miniTurbo Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-19 02:11:49 0
pJWK_VD_01
 
Resource Report
Resource Website
RRID:Addgene_158102 5'UTR synthetic stem-loop 0kcal/mol Synthetic Ampicillin PMID:32846140 Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-19 02:11:48 0
p663-UBC-miniTurbo-V5-ICK_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_158187 miniTurbo-V5-ICK Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-19 02:11:49 0
p663-UBC-miniTurbo-V5-DYRK4_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_158184 miniTurbo-V5-DYRK4 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-19 02:11:49 0
pscALPSpuro-FcACE2 (Cat)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_158082 Cat ACE2 Ampicillin PMID:32991842 Vector Backbone:pscALPS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-19 02:11:47 1

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