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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pJL2-mGold-P2A-EBFP2 Resource Report Resource Website |
RRID:Addgene_157998 | mGold-P2A-EBFP2 | Synthetic | Ampicillin | PMID:33097540 | A mammalian expression cassette was cloned into a yeast vector. mGold: FPBaseID TBD EBFP2: FPBaseID DVMQ7 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. | Backbone Size:6992; Vector Backbone:pJL2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mGold is mVenus with L46F;T63S mutations | 2026-09-19 02:11:46 | 0 |
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pJL-mGold Resource Report Resource Website |
RRID:Addgene_157997 | mGold | Synthetic | Ampicillin | PMID:33097540 | Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. | Backbone Size:6950; Vector Backbone:pJL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | mGold is mVenus with L46F;T63S mutations | 2026-09-19 02:11:46 | 0 |
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pSFFV_CloGFP(1-10) Resource Report Resource Website |
RRID:Addgene_157994 | CloGFP(1-10) | Synthetic | Ampicillin | PMID:33227014 | Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | G32Q, F46L, K101R, S202L | 2026-09-19 02:11:46 | 0 | |
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pSFFV_mNG3K(1-10) Resource Report Resource Website 1+ mentions |
RRID:Addgene_157993 | pSFFV_mNG3K(1-10) | Synthetic | Ampicillin | PMID:33227014 | Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | G27D, D42E, M128I, K153M, T170I | 2026-09-19 02:11:46 | 2 | |
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pCaggs-mGold Resource Report Resource Website 1+ mentions |
RRID:Addgene_157996 | mGold | Synthetic | Ampicillin | PMID:33097540 | Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold. | Backbone Size:6105; Vector Backbone:pCaggs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mGold is mVenus with L46F;T63S mutations | 2026-09-19 02:11:46 | 1 |
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pVL1393-MAL-SYK Resource Report Resource Website |
RRID:Addgene_157990 | Spleen tyrosine kinase | Homo sapiens | Ampicillin | PMID:29890824 | Vector Backbone:pVL1393; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:46 | 0 | ||
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pLKO-CreERT2 sgAdam10 v3 Resource Report Resource Website |
RRID:Addgene_158047 | Adam10 sgRNA | Mus musculus | Ampicillin | PMID:32165588 | Vector Backbone:pLKO-ERT2 Cre stuffer v3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:47 | 0 | ||
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pBS-GMR-eya(shRNA) Resource Report Resource Website 1+ mentions |
RRID:Addgene_157991 | Ampicillin | PMID:33016195 | Backbone Size:2961; Vector Backbone:pBSII-KS(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:46 | 3 | ||||
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pDOC-K-glmS-GFPrev Resource Report Resource Website |
RRID:Addgene_158060 | Green Fluorescent Protein optimised for excitation with UV light | Synthetic | Ampicillin | PMID:33226934 | Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:47 | 0 | ||
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pDOC-K-glmS-lacIZ Resource Report Resource Website |
RRID:Addgene_158061 | lacIZ operon | Escherichia coli | Ampicillin | PMID:33226934 | Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:47 | 0 | ||
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pDOC-K-glmS Resource Report Resource Website |
RRID:Addgene_158058 | glmS homologous region 1 | Salmonella enterica Ser. Typhimurium | Ampicillin | PMID:33226934 | Backbone Size:7233; Vector Backbone:pDOC-K; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:47 | 0 | ||
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p667-UBC-PHKG1-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_158192 | PHKG1-V5-miniTurbo | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:49 | 0 | ||
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p667-UBC-DYRK3-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_158190 | DYRK3-V5-miniTurbo | Homo sapiens | Ampicillin | Depositor confirms R293H does not affect plasmid function. This is a known sequence conflict. These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | R293H- please see depositor comments | 2026-09-19 02:11:49 | 0 | |
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pJWK_VD_02 Resource Report Resource Website |
RRID:Addgene_158103 | 5'UTR synthetic stem-loop -30kcal/mol | Synthetic | Ampicillin | PMID:32846140 | Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:48 | 0 | ||
|
pJWK_VD_03 Resource Report Resource Website |
RRID:Addgene_158104 | 5'UTR of CACNA1C (uc009zdu.1) | Homo sapiens | Ampicillin | PMID:32846140 | Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:48 | 0 | ||
|
p667-UBC-BCKDK-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_158189 | BCKDK-V5-miniTurbo | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:49 | 0 | ||
|
pJWK_VD_01 Resource Report Resource Website |
RRID:Addgene_158102 | 5'UTR synthetic stem-loop 0kcal/mol | Synthetic | Ampicillin | PMID:32846140 | Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:48 | 0 | ||
|
p663-UBC-miniTurbo-V5-ICK_IDG-K Resource Report Resource Website |
RRID:Addgene_158187 | miniTurbo-V5-ICK | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:49 | 0 | ||
|
p663-UBC-miniTurbo-V5-DYRK4_IDG-K Resource Report Resource Website |
RRID:Addgene_158184 | miniTurbo-V5-DYRK4 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:49 | 0 | ||
|
pscALPSpuro-FcACE2 (Cat) Resource Report Resource Website 1+ mentions |
RRID:Addgene_158082 | Cat ACE2 | Ampicillin | PMID:32991842 | Vector Backbone:pscALPS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:47 | 1 |
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