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Species: Human coronavirus OC43
Genetic Insert: Human coronavirus OC43 nucleocapsid protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: P33469
Proper citation: RRID:Addgene_151902 Copy
Species: Xenopus laevis
Genetic Insert: Xnr4
Vector Backbone Description: Backbone Size:3260; Vector Backbone:pSP64TNE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9133442
Comments: For use in mRNA microinjection experiments.
Proper citation: RRID:Addgene_15078 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 spike prefusion-stabilized
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: QHN73810.1
Proper citation: RRID:Addgene_151911 Copy
Genetic Insert: no gene
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS300 (modified pCS2); Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9651252
Comments: pCS300 differs from pCS2 in that the polylinker II Not I site was moved to the polylinker I area and an Asc I and Pme I site were added to the polylinker II area.
You can use the CMV promoter in tissue culture cells or Xenopus embryos.
Proper citation: RRID:Addgene_15082 Copy
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:6500; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006984
Comments: A replication-incompetent pLIA retroviral vector, based on the pBABE vector. In addition to the MMLV long terminal repeats, it also contains an internal ribosomal entry site (IRES) sequence derived from the encephalomyocarditis virus, in which the eleventh ATG of the IRES was fused in frame to the human placental alkaline phosphatase gene (PLAP).
pLIA has a cloning site for expressing exogenous genes under the transcriptional control of the LTR. The cloning site contains NotI, SfiI, SnaBI, and EcoRI.
Proper citation: RRID:Addgene_15129 Copy
Species: Mus musculus
Genetic Insert: CRX
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:6500; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390562
Comments: An EcoRV-HincII Crx cDNA fragment containing the entire Crx coding region was subcloned into the SnaBI site of the pLIA retrovirus vector.
Proper citation: RRID:Addgene_15128 Copy
Species: Mus musculus
Genetic Insert: CRX
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390562
Comments: CRX cDNA
Proper citation: RRID:Addgene_15127 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pCF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10938113
Comments: pC.HA was derived from plasmid pCG (Tanaka, M, and Herr, W., 1990. Cell 60:375-386). pCG contains the human CMV promoter, the HSV tk gene 5' untranslated leader and initiation codon, rabbit beta-globin gene splicing and polyA singals, and the replicon origin of SV40.
Proper citation: RRID:Addgene_15126 Copy
Species: Homo sapiens
Genetic Insert: alpha-globin EDB fibronectin minigene
Vector Backbone Description: Backbone Size:2960; Vector Backbone:pBlueScript II KS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12719375
Comments: Hybrid minigene used for splicing assay for pre-mRNA processing. Contains a unique NdeI site for subcloning sequence of interest.
Proper citation: RRID:Addgene_15125 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: QHN73810.1
Proper citation: RRID:Addgene_151921 Copy
Species: Homo sapiens
Genetic Insert: TRAF6 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Comments: Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.
Proper citation: RRID:Addgene_15094 Copy
Species: Homo sapiens
Genetic Insert: miR-146a promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Proper citation: RRID:Addgene_15091 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS II +; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: Contains entire ORF of BMP receptor. The insert cuts with EcoRV, HindIII, BglII, KpnI, BamHI.
Proper citation: RRID:Addgene_15059 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 spike prefusion-stabilized
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: QHN73810.1
Proper citation: RRID:Addgene_151930 Copy
Species: Xenopus laevis
Genetic Insert: Xfin fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2417; Vector Backbone:pSP70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2826129
Comments: See author's map for more information.
Proper citation: RRID:Addgene_15054 Copy
Species: Xenopus laevis
Genetic Insert: Xfin fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2417; Vector Backbone:pSP70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2826129
Comments: See author's map for more information.
Proper citation: RRID:Addgene_15053 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV Sport 6 with U6 promoter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16426460
Comments: The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.
See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).
Proper citation: RRID:Addgene_15173 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Size:12000; Vector Backbone:RCAS (A); Vector Types:Retroviral, RNAi, Avian expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16426460
Comments: The RCAS-RNAi viruses have been modified so that they are now compatible with the Gateway cloning system (Invitrogen). DNA encoding the hairpin is cloned into a modified U6 vector using standard cloning techniques. The construct is then moved into the retroviral vector using Gateway reagents, thus bypassing the difficulties of cloning small fragments into RCAS. As a result, each construct can be made with a minimal number of cloning steps. See Supplemental Information of the article for a detailed protocol.
Proper citation: RRID:Addgene_15182 Copy
Species: Mus musculus
Genetic Insert: beta2-YFP-M
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Comments: YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M).
Proper citation: RRID:Addgene_15107 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TNE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make mRNA for the full length BMP receptor. The clone contains no untranslated sequences.
Proper citation: RRID:Addgene_15068 Copy
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