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http://www.addgene.org/122497

Genetic Insert: Strep-tagged 10xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector. pStrep-1xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG pStrep-2x/…/12xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

Proper citation: RRID:Addgene_122497 Copy   

•   Source: Addgene
  

http://www.addgene.org/122496

Genetic Insert: Strep-tagged 9xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector. pStrep-1xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG pStrep-2x/…/12xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

Proper citation: RRID:Addgene_122496 Copy   

•   Source: Addgene
  

http://www.addgene.org/122499

Genetic Insert: Strep-tagged 12xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector. pStrep-1xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG pStrep-2x/…/12xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

Proper citation: RRID:Addgene_122499 Copy   

•   Source: Addgene
  

http://www.addgene.org/122535

Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2_lmp-1::CemOrange2
Vector Backbone Description: Vector Backbone:pPD49.26; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30913273

Proper citation: RRID:Addgene_122535 Copy   

•   Source: Addgene
  

http://www.addgene.org/122529

Genetic Insert: Tobacco etch virus cDNA fragment 3
Vector Backbone Description: Vector Backbone:p15A; Vector Types:potyvirus vectors to produce carotenoids in plants; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31745931

Proper citation: RRID:Addgene_122529 Copy   

•   Source: Addgene
  

http://www.addgene.org/122528

Genetic Insert: Tobacco etch virus cDNA fragment
Vector Backbone Description: Vector Backbone:p15A; Vector Types:potyvirus vectors to produce carotenoids in plants; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31745931

Proper citation: RRID:Addgene_122528 Copy   

•   Source: Addgene
  

http://www.addgene.org/122480

Species: Homo sapiens
Genetic Insert: IFITM3
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30643282
Comments: Please note that this plasmid does not contain an HA tag.

Proper citation: RRID:Addgene_122480 Copy   

•   Source: Addgene
  

http://www.addgene.org/122482

Species: Homo sapiens
Genetic Insert: isocitrate dehydrogenase 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7725; Vector Backbone:pLenti/TO/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29288440

Proper citation: RRID:Addgene_122482 Copy   

•   Source: Addgene
  

http://www.addgene.org/1225

Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Size:6464; Vector Backbone:pRS306; Vector Types:yeast integrative plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12461168

Proper citation: RRID:Addgene_1225 Copy   

•   Source: Addgene
  

http://www.addgene.org/122484

Species: Drosophila melanogaster
Genetic Insert: Kinesin 1-401 iLid His tag
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31391558

Proper citation: RRID:Addgene_122484 Copy   

•   Source: Addgene
  

http://www.addgene.org/122486

Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pUASg; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28761084

Proper citation: RRID:Addgene_122486 Copy   

•   Source: Addgene
  

http://www.addgene.org/122488

Genetic Insert: Strep-tagged 1xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector. pStrep-1xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG pStrep-2x/…/12xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

Proper citation: RRID:Addgene_122488 Copy   

•   Source: Addgene
  

http://www.addgene.org/122523

Species: Rattus norvegicus
Genetic Insert: eGFP-SynaptoZip
Vector Backbone Description: Backbone Marker:L Naldini; Backbone Size:7085; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29089485

Proper citation: RRID:Addgene_122523 Copy   

•   Source: Addgene
  

http://www.addgene.org/122489

Genetic Insert: Strep-tagged 2xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31521668
Comments: Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector. pStrep-1xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG pStrep-2x/…/12xEGFP-N1: G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

Proper citation: RRID:Addgene_122489 Copy   

•   Source: Addgene
  

http://www.addgene.org/122522

Species: Escherichia virus T4
Genetic Insert: gp43 - DNA polymerase
Vector Backbone Description: Backbone Size:4675; Vector Backbone:pETMCSI; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30673756

Proper citation: RRID:Addgene_122522 Copy   

•   Source: Addgene
  

http://www.addgene.org/12248

Genetic Insert: shRNA against GFP
Vector Backbone Description: Backbone Size:10242; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12248 Copy   

•   Source: Addgene
  

http://www.addgene.org/12247

Vector Backbone Description: Backbone Size:11087; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab. pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI. Please note that there two additional Dam-methylase-sensitive ClaI sites in this vector that are blocked by Dam methylation (this does not appear in the depositor's full sequence) around the EF1a promoter. The plasmid needs to be grown in a dam(-) bacterial strain in order to use ClaI for cloning this region. Otherwise, dam(+) strains can be used for MluI-ClaI cloning shRNA. Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work). Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid). The packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12247 Copy   

•   Source: Addgene
  

http://www.addgene.org/122518

Genetic Insert: iCre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_122518 Copy   

•   Source: Addgene
  

http://www.addgene.org/12249

Genetic Insert: TetR-KRAB fusion
Vector Backbone Description: Backbone Size:10713; Vector Backbone:pLV-tTRKRAB; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: Older, double-copy vector system for conditional expression of siRNA. Has been replaced by the all-in-one vector system from Trono/Aebischer lab (e.g. see Addgene plasmid 11643). For conditional expression of siRNA, the target cells need to be cotransduced with both pLVTH/pLVTHM and pLV-tTRKRAB/pLV-tTRKRAB-Red to achieve conditional expression of siRNA. Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12249 Copy   

•   Source: Addgene
  

http://www.addgene.org/122475

Species: Homo sapiens
Genetic Insert: IFITM3
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30643282

Proper citation: RRID:Addgene_122475 Copy   

•   Source: Addgene