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Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:1731342
Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
Proper citation: RRID:Addgene_15164 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus activin receptor 1
Vector Backbone Description: Backbone Size:3260; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1328888
Comments: For expression in embryos. Note that this construct does not have a 5' UTR.
Proper citation: RRID:Addgene_15042 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:12000; Vector Backbone:RCASBP (E); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8455633
Comments: In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(E) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
Proper citation: RRID:Addgene_15162 Copy
Species: Gallus gallus
Genetic Insert: Irx4
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10024241
Comments: IRX4 cDNA
Proper citation: RRID:Addgene_15139 Copy
Species: Mus musculus
Genetic Insert: Rax
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096350
Comments: Rax cDNA
Proper citation: RRID:Addgene_15138 Copy
Species: Gallus gallus
Genetic Insert: GH6
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060230
Proper citation: RRID:Addgene_15137 Copy
Genetic Insert: nLacZ
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pRAVE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12413898
Comments: In addition to the long terminal repeat (LTR) promoter, the RAVE-nlacZ viral
vector has an internal Xenopus elongation factor alpha (EF1-alpha) promoter. To construct RAVE-nlacZ, the Xenopus EF1-alpha gene from pEF-puro (from Gord Fishell), was cloned into the pRIA vector.
Proper citation: RRID:Addgene_15135 Copy
Vector Backbone Description: Backbone Size:4835; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD99.44, Ligation number L2680.
Proper citation: RRID:Addgene_1516 Copy
Species: Rattus norvegicus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Gary Nolan, Stanford; Backbone Size:0; Vector Backbone:pBABE-MN-IRES-E-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839357
Comments: An EcoRI-AhaIII fragment containing the entire Hes1 coding region was blunted and subcloned in the blunted BamHI site of the pGFP virus vector.
Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_15133 Copy
Genetic Insert: PGL3 luciferase siRNA
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs
See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case.
Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.
Proper citation: RRID:Addgene_15099 Copy
Species: Gallus gallus
Genetic Insert: SOHo1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060230
Proper citation: RRID:Addgene_15132 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
Proper citation: RRID:Addgene_15098 Copy
Species: Mus musculus
Genetic Insert: Notch1 (C-term)
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006984
Comments: mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.
Proper citation: RRID:Addgene_15131 Copy
Species: Homo sapiens
Genetic Insert: IRAK1 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Comments: Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of IRAK1 with 4-bp substitutions in the miR-146 target sites.
Proper citation: RRID:Addgene_15096 Copy
Vector Backbone Description: Backbone Size:4919; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.78, Ligation number L2603.
Proper citation: RRID:Addgene_1511 Copy
Vector Backbone Description: Backbone Size:4702; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.82, Ligation number L2604.
Proper citation: RRID:Addgene_1512 Copy
Species: Escherichia coli
Genetic Insert: beta-galactosidase
Vector Backbone Description: Backbone Size:0; Vector Backbone:modified pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:3099292
Comments: Retroviral vector expressing beta-galactosidase from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Proper citation: RRID:Addgene_15148 Copy
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:9795; Vector Backbone:pRIAS; Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525341
Comments: A replication-incompetent avian retroviral vector for transgene expression. Clone your transgene into pSLIRES11 and use ClaI to transfer it to pRIAS.
Proper citation: RRID:Addgene_15147 Copy
Vector Backbone Description: Backbone Size:8123; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD96.62, Ligation number NONE.
Proper citation: RRID:Addgene_1505 Copy
Vector Backbone Description: Backbone Size:0; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD54.01, Ligation number pPD54_01.
Proper citation: RRID:Addgene_1507 Copy
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