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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
DAP (DOL-AP) (CC#44)
 
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RRID:Addgene_15164 PLAP Homo sapiens Kanamycin PMID:1731342 Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter. Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL. Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin 2026-09-19 02:10:52 1
pSP64T XAR1 DNM1 (DM#102)
 
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RRID:Addgene_15042 Xenopus activin receptor 1 Xenopus laevis Ampicillin PMID:1328888 For expression in embryos. Note that this construct does not have a 5' UTR. Backbone Size:3260; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Dominant negative mutant 2026-09-19 02:10:51 0
RCASBP-AP (E) (CC#39)
 
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RRID:Addgene_15162 PLAP Homo sapiens Ampicillin PMID:8455633 In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(E) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3'). Backbone Size:12000; Vector Backbone:RCASBP (E); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
pBS chick IRX4 (CC#7)
 
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RRID:Addgene_15139 Irx4 Gallus gallus Ampicillin PMID:10024241 IRX4 cDNA Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
pBS Rax (CC#5)
 
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RRID:Addgene_15138 Rax Mus musculus Ampicillin PMID:9096350 Rax cDNA Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
RCAS GH6 (CC#152)
 
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RRID:Addgene_15137 GH6 Gallus gallus Ampicillin PMID:11060230 Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
pRAVE-nLacZ (CC#111)
 
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RRID:Addgene_15135 nLacZ Ampicillin PMID:12413898 In addition to the long terminal repeat (LTR) promoter, the RAVE-nlacZ viral vector has an internal Xenopus elongation factor alpha (EF1-alpha) promoter. To construct RAVE-nlacZ, the Xenopus EF1-alpha gene from pEF-puro (from Gord Fishell), was cloned into the pRIA vector. Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pRAVE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
L2680
 
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1+ mentions
RRID:Addgene_1516 Ampicillin See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD99.44, Ligation number L2680. Backbone Size:4835; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 1
pHes1-GFP (CC#109)
 
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RRID:Addgene_15133 Hes1 Rattus norvegicus Ampicillin PMID:10839357 An EcoRI-AhaIII fragment containing the entire Hes1 coding region was blunted and subcloned in the blunted BamHI site of the pGFP virus vector. Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow. Backbone Marker:Gary Nolan, Stanford; Backbone Size:0; Vector Backbone:pBABE-MN-IRES-E-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
V45 pHIPPY PGL3 Luciferase
 
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RRID:Addgene_15099 PGL3 luciferase siRNA Bleocin (Zeocin) PMID:15119963 Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case. Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence. Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin) 2026-09-19 02:10:52 0
pBS SOHo1 (CC#99)
 
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RRID:Addgene_15132 SOHo1 Gallus gallus Ampicillin PMID:11060230 Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
V44 pHIPPY blunt
 
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RRID:Addgene_15098 Bleocin (Zeocin) PMID:15119963 This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells. See article and author's map for more information. Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin) 2026-09-19 02:10:52 0
pLIA mNIC myc (CC#341)
 
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1+ mentions
RRID:Addgene_15131 Notch1 (C-term) Mus musculus Ampicillin PMID:9006984 mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus. Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Cytoplasmic domain initiating at amino acid 1753. See author's map and article for more information. 2026-09-19 02:10:52 5
IRAK1mut-UTR
 
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1+ mentions
RRID:Addgene_15096 IRAK1 3'UTR Homo sapiens Ampicillin PMID:16885212 Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of IRAK1 with 4-bp substitutions in the miR-146 target sites. Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 4-bp substitutions in the miR-146 target sites 2026-09-19 02:10:52 3
L2603
 
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1+ mentions
RRID:Addgene_1511 Ampicillin See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.78, Ligation number L2603. Backbone Size:4919; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 1
L2604
 
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1+ mentions
RRID:Addgene_1512 Ampicillin See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD97.82, Ligation number L2604. Backbone Size:4702; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 1
BAG (CC#23)
 
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RRID:Addgene_15148 beta-galactosidase Escherichia coli Kanamycin PMID:3099292 Retroviral vector expressing beta-galactosidase from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter. Backbone Size:0; Vector Backbone:modified pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin 2026-09-19 02:10:52 0
pRIAS (CC#15)
 
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RRID:Addgene_15147 Ampicillin PMID:10525341 A replication-incompetent avian retroviral vector for transgene expression. Clone your transgene into pSLIRES11 and use ClaI to transfer it to pRIAS. Backbone Marker:Cepko Lab; Backbone Size:9795; Vector Backbone:pRIAS; Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 0
pPD96_62
 
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RRID:Addgene_1505 Ampicillin See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD96.62, Ligation number NONE. Backbone Size:8123; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 0
pPD54_01
 
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1+ mentions
RRID:Addgene_1507 Ampicillin See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD54.01, Ligation number pPD54_01. Backbone Size:0; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:52 1

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