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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pSP64TS Activin pro Vg1 Flag (DM#171) Resource Report Resource Website |
RRID:Addgene_15010 | activin-Vg1 | Xenopus laevis | Ampicillin | PMID:8797821 | Chimeric activin-Vg1 mRNA, encoding the pro-region of activin βB fused to the mature region of Vg1, resulting in the processing and secretion of mature Vg1. The activin βB-Vg1 hybrid was constructed by PCR amplification of a fragment of a Xenopus activin βB gene encoding the N-terminal pro-region, the tetrabasic cleavage site and the first four amino acids of the C-terminal mature region. This fragment was ligated, in frame, to a PCR amplified fragment of the Vg1 gene encoding the C-terminal mature region lacking the first four amino acids. The ligation product was reamplified, digested at terminal restriction sites and cloned into pSP64TS. The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site. See Author's Map for more information. | Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | |
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pBbdCas9S_P0526-sgRNAmreB Resource Report Resource Website |
RRID:Addgene_149654 | dCas9, lacI, sgRNAmreB | Synthetic | Spectinomycin | PMID:33257311 | Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-09-19 02:10:50 | 0 | ||
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pBbdCas9S_Psyn-sgRNArodA Resource Report Resource Website |
RRID:Addgene_149655 | dCas9, lacI, sgRNArodA | Synthetic | Spectinomycin | PMID:33257311 | Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-09-19 02:10:50 | 0 | ||
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pBbdCas9S_P0526-sgRNArodA Resource Report Resource Website |
RRID:Addgene_149658 | dCas9, lacI, sgRNArodA | Synthetic | Spectinomycin | PMID:33257311 | Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-09-19 02:10:50 | 0 | ||
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pBV-Luc/Del-6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_14969 | c-myc promoter (-109/+334) | Homo sapiens | Ampicillin | PMID:11158583 | XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid. | Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | -109/+334 (relative to the P2 transcription initiation site) of the c-myc promoter. | 2026-09-19 02:10:50 | 1 |
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pCINeo-hFX(I154F)-HA Resource Report Resource Website |
RRID:Addgene_14977 | human frataxin cDNA | Homo sapiens | Ampicillin | PMID:16263703 | Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:PCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed isoleucine 154 to phenylalanine | 2026-09-19 02:10:51 | 0 | |
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pMSCV TbetaRII ecto Resource Report Resource Website |
RRID:Addgene_14974 | TbetaRII ecto | Homo sapiens | Ampicillin | PMID:16286245 | Soluble TbetaRII. See author's map for more information. | Backbone Size:6300; Vector Backbone:pMSCV Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | ecto domain | 2026-09-19 02:10:51 | 0 |
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Hsp68-CreER(TM) (DM#264) Resource Report Resource Website 1+ mentions |
RRID:Addgene_15028 | Cre-ER(TM) | Ampicillin | The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. See Author's Map for more information. | Backbone Size:3000; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 1 | |||
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pBS mouse c-Myc Resource Report Resource Website |
RRID:Addgene_14972 | c-Myc | Mus musculus | Ampicillin | PMID:12824180 | See author's map for more information. | Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Nucleotides 2578-3294 | 2026-09-19 02:10:51 | 0 |
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TOPOII p48 (DM#261) Resource Report Resource Website |
RRID:Addgene_15026 | p48 | Mus musculus | Ampicillin | To make antisense probe: Cut with NotI and transcribe with Sp6 polymerase. | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | ||
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TOPOII Pdx1 (DM#260) Resource Report Resource Website |
RRID:Addgene_15025 | Pdx1 | Mus musculus | Ampicillin | To make antisense probe: Cut with NotI and transcribe with Sp6 polymerase. | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | ||
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Ngn3 Cre-ER (DM#256) Resource Report Resource Website |
RRID:Addgene_15024 | Ngn3 promoter | Mus musculus | Ampicillin | PMID:11973276 | The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library (Genetics Institute, Buffalo, NY). Three primers were used to fuse the Cre-ERTM-coding region to the Ngn3 ATG to generate a fragment that had part of the Ngn3 promoter and the 5' end of the recombinase. The primers used are p73 (5'-acacttgactccttgatcgctg-3'), p71 (5'-gttgcatcgaccggtaatgca-3') and p74 (5'-ttttcccaaccgcaggatgtccaatttactgaccgtacac-3'). This fragment was subsequently digested with ApaI and AgeI and ligated to the KpnI (blunt-ended)-ApaI (partial digestion)-digested pGD15, and a 2.4 kb AgeI-SacII (partial digestion, blunt-ended) fragment of pGD35. The insert from the final construct (pGD29) was released by NotI digestion. See Author's Map and article for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | |
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Ngn3 Cre (DM#259) Resource Report Resource Website |
RRID:Addgene_15023 | Ngn3 promoter | Mus musculus | Ampicillin | PMID:11973276 | To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production. See Author's Map and article for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | |
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Pdx1 Cre-ER (DM#255) Resource Report Resource Website |
RRID:Addgene_15022 | Pdx1 promoter | Mus musculus | Ampicillin | PMID:11973276 | The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. For Pdx-1-Cre-ER(TM), the coding region of the Cre-ER(TM) cDNA was directly fused to the starting ATG of the PDX1 protein by PCR. Three primers (p5, 5'-ttgaaacaagtgcaggtgttcg-3'; p75, 5'-gttgcatcgaccggtaatgcaggcaaattttggtgtacggtcagtaaattggacatggtggcagccggcact-3'; and p71, 5'-gttgcatcgaccggtaatgca-3') were used. First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) plasmid to obtain a fragment that has the 5' end coding region directly fused to the Pdx1 promoter. This fragment was digested with AgeI and ligated to the XhoI (blunt-ended)-AgeI double-digested pBSCre-ER(TM) that contains the full-length Cre-ER-coding region to give pGD19. Then a 2.2 kb insert was released from pGD19 by SmaI-SpeI digestion and ligated to SmaI-NotI digested pKSpdx-1SalI, and a SpeI-NotI digested PCR fragment that contains the SV40 polyadenylation signal to give pGD35. The 8 kb insert was released by SalI-NotI digestion and was used for pronucleus injection. See Author's Map and article for more information. SalI/SmaI digest should give 6.7, 1.5, 4 kb bands. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 0 | |
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Pdx1 Cre (DM#258) Resource Report Resource Website 1+ mentions |
RRID:Addgene_15021 | Pdx1 promoter | Mus musculus | Ampicillin | PMID:11973276 | To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion. See Author's Map for more information. | Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin | 2026-09-19 02:10:51 | 1 | |
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pBbdCas9S(-10AC2)_Psyn-sgRNArodA Resource Report Resource Website |
RRID:Addgene_149660 | dCas9, lacI, sgRNArodA | Synthetic | Spectinomycin | PMID:33257311 | Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10AC2); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-09-19 02:10:50 | 0 | ||
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pcDNA3-sACE2v2.2-8his Resource Report Resource Website 1+ mentions |
RRID:Addgene_149663 | Angiotensin-converting enzyme 2 | Homo sapiens | Ampicillin | PMID:32753553 | Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. | Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, N330Y, A386L | 2026-09-19 02:10:50 | 1 |
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pcDNA3-sACE2v2.2-sfGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_149665 | Angiotensin-converting enzyme 2 | Homo sapiens | Ampicillin | PMID:32753553 | Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. | Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, N330Y, A386L | 2026-09-19 02:10:50 | 1 |
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pcDNA3-sACE2v2.4-IgG1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_149668 | Angiotensin-converting enzyme 2 | Homo sapiens | Ampicillin | PMID:32753553 | Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint. | Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Extracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, L79T, N330Y | 2026-09-19 02:10:50 | 1 |
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pSCALPS_miR-146aSponge_mCherry_GFP Resource Report Resource Website |
RRID:Addgene_149718 | 14X miR-146a binding sites | Synthetic | Ampicillin | PMID:32205878 | Backbone Size:8518; Vector Backbone:pSCALPS_mCherry_GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | G to T mutation in position 75 of the insert | 2026-09-19 02:10:51 | 0 |
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