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Species: Xenopus laevis
Genetic Insert: activin-Vg1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8797821
Comments: Chimeric activin-Vg1 mRNA, encoding the pro-region of activin βB fused to the mature region of Vg1, resulting in the processing and secretion of mature Vg1.
The activin βB-Vg1 hybrid was constructed by PCR amplification of a fragment of a Xenopus activin βB gene encoding the N-terminal pro-region, the tetrabasic cleavage site and the first four amino acids of the C-terminal mature region. This fragment was ligated, in frame, to a PCR amplified fragment of the Vg1 gene encoding the C-terminal mature region lacking the first four amino acids. The ligation product was reamplified, digested at terminal restriction sites and cloned into pSP64TS.
The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site.
See Author's Map for more information.
Proper citation: RRID:Addgene_15010 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149654 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149655 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149658 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (-109/+334)
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158583
Comments: XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid.
Proper citation: RRID:Addgene_14969 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:PCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16263703
Proper citation: RRID:Addgene_14977 Copy
Species: Homo sapiens
Genetic Insert: TbetaRII ecto
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCV Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16286245
Comments: Soluble TbetaRII. See author's map for more information.
Proper citation: RRID:Addgene_14974 Copy
Genetic Insert: Cre-ER(TM)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
See Author's Map for more information.
Proper citation: RRID:Addgene_15028 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12824180
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14972 Copy
Species: Mus musculus
Genetic Insert: p48
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To make antisense probe: Cut with NotI and transcribe with Sp6 polymerase.
Proper citation: RRID:Addgene_15026 Copy
Species: Mus musculus
Genetic Insert: Pdx1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To make antisense probe: Cut with NotI and transcribe with Sp6 polymerase.
Proper citation: RRID:Addgene_15025 Copy
Species: Mus musculus
Genetic Insert: Ngn3 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library (Genetics Institute, Buffalo, NY). Three primers were used to fuse the Cre-ERTM-coding region to the Ngn3 ATG to generate a fragment that had part of the Ngn3 promoter and the 5' end of the recombinase. The primers used are p73 (5'-acacttgactccttgatcgctg-3'), p71 (5'-gttgcatcgaccggtaatgca-3') and p74 (5'-ttttcccaaccgcaggatgtccaatttactgaccgtacac-3'). This fragment was subsequently digested with ApaI and AgeI and ligated to the KpnI (blunt-ended)-ApaI (partial digestion)-digested pGD15, and a 2.4 kb AgeI-SacII (partial digestion, blunt-ended) fragment of pGD35. The insert from the final construct (pGD29) was released by NotI digestion.
See Author's Map and article for more information.
Proper citation: RRID:Addgene_15024 Copy
Species: Mus musculus
Genetic Insert: Ngn3 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production.
See Author's Map and article for more information.
Proper citation: RRID:Addgene_15023 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
For Pdx-1-Cre-ER(TM), the coding region of the Cre-ER(TM) cDNA was directly fused to the starting ATG of the PDX1 protein by PCR. Three primers (p5, 5'-ttgaaacaagtgcaggtgttcg-3'; p75, 5'-gttgcatcgaccggtaatgcaggcaaattttggtgtacggtcagtaaattggacatggtggcagccggcact-3'; and p71, 5'-gttgcatcgaccggtaatgca-3') were used. First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) plasmid to obtain a fragment that has the 5' end coding region directly fused to the Pdx1 promoter. This fragment was digested with AgeI and ligated to the XhoI (blunt-ended)-AgeI double-digested pBSCre-ER(TM) that contains the full-length Cre-ER-coding region to give pGD19. Then a 2.2 kb insert was released from pGD19 by SmaI-SpeI digestion and ligated to SmaI-NotI digested pKSpdx-1SalI, and a SpeI-NotI digested PCR fragment that contains the SV40 polyadenylation signal to give pGD35. The 8 kb insert was released by SalI-NotI digestion and was used for pronucleus injection.
See Author's Map and article for more information.
SalI/SmaI digest should give 6.7, 1.5, 4 kb bands.
Proper citation: RRID:Addgene_15022 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.
See Author's Map for more information.
Proper citation: RRID:Addgene_15021 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10AC2); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149660 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149663 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149665 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_149668 Copy
Species: Synthetic
Genetic Insert: 14X miR-146a binding sites
Vector Backbone Description: Backbone Size:8518; Vector Backbone:pSCALPS_mCherry_GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205878
Proper citation: RRID:Addgene_149718 Copy
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