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Species: Saccharomyces cerevisiae
Genetic Insert: Ty3-his3AI
Vector Backbone Description: Vector Backbone:pIBI20; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26421679
Proper citation: RRID:Addgene_113390 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11338 Copy
Species: Homo sapiens
Genetic Insert: rs281038 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113371 Copy
Species: Homo sapiens
Genetic Insert: rs67668514 minor allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113376 Copy
Species: Homo sapiens
Genetic Insert: rs67668514 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113375 Copy
Species: Homo sapiens
Genetic Insert: rs2941742 minor allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113374 Copy
Species: Homo sapiens
Genetic Insert: rs2149943 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113379 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Ras1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7923372
Comments: cDNA of fission yeast S. pombe Ras1 gene (oncogene) cloned into the yeast two-hybrid DNA-binding domain vector pLexA (ref., Chang et al., 2004, Cell, 79:131-141). See "author's map" for picture of empty pLexA vector.
Proper citation: RRID:Addgene_11340 Copy
Species: Homo sapiens
Genetic Insert: rs62288111 minor allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113360 Copy
Species: Homo sapiens
Genetic Insert: rs34466261 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113365 Copy
Species: Homo sapiens
Genetic Insert: rs7007731 minor allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113362 Copy
Species: Homo sapiens
Genetic Insert: rs11716984 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113367 Copy
Species: unknown
Genetic Insert: CD28-PafA
Vector Backbone Description: Vector Backbone:pEF6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104635
Proper citation: RRID:Addgene_113400 Copy
Species: Homo sapiens
Genetic Insert: PVT1-enhancer
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113350 Copy
Species: Homo sapiens
Genetic Insert: ATP9A-enhancer
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113354 Copy
Species: Homo sapiens
Genetic Insert: KAT6B-enhancer
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113353 Copy
Species: Homo sapiens
Genetic Insert: QSOX1-enhancer
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113352 Copy
Species: Homo sapiens
Genetic Insert: rs6990531 minor allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113358 Copy
Species: Homo sapiens
Genetic Insert: rs7957274 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113355 Copy
Genetic Insert: 2 binding sites for let-7a miRNA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.
Proper citation: RRID:Addgene_11327 Copy
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