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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBbdCas9B(-10TC)_arr2
 
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RRID:Addgene_149586 Blasticidin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9B(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Blasticidin 2026-09-19 02:10:49 0
pBbdCas9H(-10TC)_arr2
 
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RRID:Addgene_149587 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-19 02:10:49 0
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
 
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RRID:Addgene_149588 dCas9, lacI, sgRNAflaB Synthetic Spectinomycin PMID:33257311 Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation. Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-09-19 02:10:49 0
pBSV2G_P0526-sgRNA500
 
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RRID:Addgene_149621 Gentamicin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-09-19 02:10:50 0
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
 
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RRID:Addgene_149589 dCas9, lacI, sgRNAflaB Synthetic Spectinomycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10TC); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-09-19 02:10:49 0
pBSV2G_P0826L-sgRNAflaB
 
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RRID:Addgene_149627 sgRNAflaB Synthetic Gentamicin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-09-19 02:10:50 0
pBSV2G_P0026-sgRNAflaB
 
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RRID:Addgene_149628 sgRNAflaB Synthetic Gentamicin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-09-19 02:10:50 0
pBSV2G_P0526-sgRNAflaB
 
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RRID:Addgene_149629 sgRNAflaB Synthetic Gentamicin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin 2026-09-19 02:10:50 0
pBlue-hFX(I154F)-HA
 
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RRID:Addgene_14988 human frataxin cDNA Homo sapiens Ampicillin Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed isoleucine 154 to phenylalanine 2026-09-19 02:10:51 0
pBlue-hFX(T133A)-HA
 
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RRID:Addgene_14989 human frataxin cDNA Homo sapiens Ampicillin Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed threonine 133 to alanine 2026-09-19 02:10:51 0
pBlue-hFX-HA
 
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RRID:Addgene_14987 human frataxin cDNA Homo sapiens Ampicillin PMID:11929854 Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 0
pBTG (pBigT-IRES-GFP; DM#268)
 
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1+ mentions
RRID:Addgene_15037 Ampicillin PMID:14657333 Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information. Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information. Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 7
pSP64T (DM#111)
 
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1+ mentions
RRID:Addgene_15030 beta-globin 5' and 3' UTR Xenopus laevis Ampicillin PMID:6207484 Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information. Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 2
pBABEpuro HA Brca1
 
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10+ mentions
RRID:Addgene_14999 Brca1 Homo sapiens Ampicillin PMID:10550055 pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro. The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate. Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 14
pIRES2-EGFP Mer
 
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1+ mentions
RRID:Addgene_14998 Mer Mus musculus Kanamycin PMID:15673687 The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above. Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-19 02:10:51 2
pET28(a)-hFX(I154F)-HA
 
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RRID:Addgene_14992 human frataxin cDNA Homo sapiens Kanamycin Backbone Marker:BMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin contains cDNA coding sequence for amino acids 88-210 of the original human frataxin gene; changed isoleucine 154 to phenylalanine (mutation I154F localization corresponds to full-length protein) 2026-09-19 02:10:51 0
pCMV5 Flag-Smad3 (146-425)
 
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1+ mentions
RRID:Addgene_14966 Smad3 Homo sapiens Ampicillin PMID:12150994 See author's map for more information. Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa 146-425 2026-09-19 02:10:50 1
pCMV5 Flag-Smad3 (220-425)
 
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1+ mentions
RRID:Addgene_14967 Smad3 Homo sapiens Ampicillin PMID:12150994 See author's map for more information. Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa 220-425 2026-09-19 02:10:50 1
RCAS chick Pax6 (DM#226)
 
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RRID:Addgene_15016 Pax6 Gallus gallus Ampicillin PMID:10906780 Backbone Size:11500; Vector Backbone:RCAS (A); Vector Types:Retroviral, avian; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 0
pSP64TS porcine TGFbeta1 (DM#167)
 
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RRID:Addgene_15011 TGFb type 1 porcine (Sus scrofa) Ampicillin PMID:8797821 The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site. See Author's Map for more information. Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin 2026-09-19 02:10:51 0

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