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Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:de Lorenzo´s lab; Vector Backbone:pSEVA448; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122096 Copy
Species: Synthetic
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122099 Copy
Genetic Insert: dCas9-10X GCN4-P2A-mCherry
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318302
Proper citation: RRID:Addgene_122132 Copy
Species: Synthetic
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122098 Copy
Genetic Insert: B. subtilis Rex
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122134 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122135 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122138 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122137 Copy
Species: Synthetic
Genetic Insert: NmCas9 tracrRNA
Vector Backbone Description: Backbone Size:4314; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122091 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: SpATG101
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976798
Proper citation: RRID:Addgene_122081 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-TERF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122084 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-LaminB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122083 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122086 Copy
Species: Synthetic
Genetic Insert: SpCas9 tracr
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122089 Copy
Species: Mus musculus
Genetic Insert: Myocardin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Comments: Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
Proper citation: RRID:Addgene_122122 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30973873
Proper citation: RRID:Addgene_122071 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25998554
Proper citation: RRID:Addgene_122073 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25998554
Comments: Depositor notes that the D308G and H545Y substitutions found in ATG13, are not of concern.
Proper citation: RRID:Addgene_122077 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122115 Copy
Species: Homo sapiens
Genetic Insert: Brg1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Proper citation: RRID:Addgene_122117 Copy
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