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Species: Homo sapiens
Genetic Insert: FMR1
Vector Backbone Description: Backbone Size:13450; Vector Backbone:Addgene #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32179589
Proper citation: RRID:Addgene_157783 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme UbE2C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4900; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17597759
Proper citation: RRID:Addgene_15779 Copy
Species: Homo sapiens
Genetic Insert: Sec16L
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFP-c1 ( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Comments: Please note that we have identified a K466R missense mutation in the Sec16L/Sec16A region of this plasmid, and the start codon may not have been correctly identified when constructing this plasmid which may have resulted in the gene being truncated. However, we do not think that this should impact plasmid functionality.
Proper citation: RRID:Addgene_15776 Copy
Species: Homo sapiens
Genetic Insert: Sec16S
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4731; Vector Backbone:pmGFPc1( modified from pEGFP-c1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17192411
Proper citation: RRID:Addgene_15775 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp14-HF
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157719 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp13-HF_GC3opt
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157716 Copy
Species: SARS-CoV-2
Genetic Insert: FH-Nsp14
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157718 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp13-HF_GC3opt
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157714 Copy
Species: Homo sapiens
Genetic Insert: H1.4ΔCTD
Vector Backbone Description: Vector Backbone:modified pMal (MTTH); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Proper citation: RRID:Addgene_157797 Copy
Species: Homo sapiens
Genetic Insert: long BRD4
Vector Backbone Description: Vector Backbone:pAV5b; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Proper citation: RRID:Addgene_157792 Copy
Species: Synthetic
Genetic Insert: 12x601_45bp linker
Vector Backbone Description: Vector Backbone:pWM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: occasional plasmid instability in dam-/dcm- strains
High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157791 Copy
Species: Homo sapiens
Genetic Insert: sfGFP-TetR
Vector Backbone Description: Vector Backbone:pETduet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Proper citation: RRID:Addgene_157794 Copy
Species: Homo sapiens
Genetic Insert: p300HAT
Vector Backbone Description: Vector Backbone:pETduet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31543265
Comments: ySIR2 required for expression & purification - A synthetic ORF encoding the histone deacetylase domain of S. cerevisiae protein SIR2 was amplified from a dsDNA synthesized by IDT using PCR and primers adding a 5'-proximal translation start codon and a 3'-proximal translation stop codon and XhoI restriction endonuclease recognition site. XhoI restriction endonuclease (NEB) digested PCR product encoding amino acids 87-562 of wild-type SIR2 was cloned into the pETduet-1 expression vector (Novagen) using a XhoI and a blunted NdeI restriction endonuclease digestion site (NEB).
Proper citation: RRID:Addgene_157793 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme UbE2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4900; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17597759
Proper citation: RRID:Addgene_15781 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme UbE2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4900; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17597759
Proper citation: RRID:Addgene_15780 Copy
Species: SARS-CoV-2
Genetic Insert: Orf6
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157726 Copy
Species: SARS-CoV-2
Genetic Insert: Nprot
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157728 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp16
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157723 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp15-HF
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157722 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp16-HF
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157725 Copy
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