Plasmids   Select Another Resource Report Type

http://www.addgene.org/105688

Species: Synthetic
Genetic Insert: P2A-3xFLAG-VHPbeta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28065601

Proper citation: RRID:Addgene_105688 Copy   

•   Source: Addgene

http://www.addgene.org/105689

Species: Synthetic
Genetic Insert: P2A-3xFLAG-VHPbeta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28065601

Proper citation: RRID:Addgene_105689 Copy   

•   Source: Addgene

http://www.addgene.org/105936

Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_105936 Copy   

•   Source: Addgene

http://www.addgene.org/105937

Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238

Proper citation: RRID:Addgene_105937 Copy   

•   Source: Addgene

http://www.addgene.org/105814

Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, RNAi, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK-RNAi series which belongs to the pKK vector family. It enables concomitant expression of two genes: 1) a cassette encoding miRNAs that target the gene of interest, and 2) an allele of the gene of interest with the protein coding sequence harbouring silent mutations that make the mRNA insensitive to the miRNAs. As a result, the endogenous alleles of the gene of interest are silenced, whereas the exogenous copy is expressed. Furthermore, the miRNAs are cotranscriptionally expressed with a fluorescent protein reporter so that analysis can be narrowed down to cells that express miRNA, provided that applied assay can distinguish cells expressing reporter. The reporter of miRNA expression has 3 repeats of the nuclear localisation signal of SV40 large T antigen, thus, it localizes to nucleus. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system. Transcription of cloned DNA is driven by a tetracycline responsive bidirectional CMV promoter (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101).

Proper citation: RRID:Addgene_105814 Copy   

•   Source: Addgene

http://www.addgene.org/105932

Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Vector Backbone:pcDNA4/TO/myc/HIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23629654

Proper citation: RRID:Addgene_105932 Copy   

•   Source: Addgene

http://www.addgene.org/105780

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mRuby3 is brighter than mCherry and has shifted excitation and emission spectra. The mClover3-mRuby3 pair is superior to EGFP-mCherry in FRET analysis. At the time of publication (PMID: 26879144) mRuby3 was the brightest coral-derived RFP in mammalian cells. mRuby3 coding sequence was cloned from Addgene ID: 74252.

Proper citation: RRID:Addgene_105780 Copy   

•   Source: Addgene

http://www.addgene.org/105788

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. Protein A of Staphylococcus aureus binds to IgG of different origin which can be used for affinity purification or antibody-based detection. Protein A (i.e. two IgG-binding domains of protein A) is often used for tandem affinity purification, in which a protein of interest is expressed in fusion with two different tags (for example Protein A-calmodulin binding peptide) (PMID: 3507693, 10504710). In this vector only Protein A is encoded.

Proper citation: RRID:Addgene_105788 Copy   

•   Source: Addgene

http://www.addgene.org/105871

Vector Backbone Description: Backbone Marker:Simpson lab; Vector Backbone:pEMS2001; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

Proper citation: RRID:Addgene_105871 Copy   

•   Source: Addgene

http://www.addgene.org/105804

Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: Addgene has found the VN173 mutation (I152L) is not present in this plasmid. This vector is a member of the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101). pKK-BiFC-Venus vector encodes fragments of Venus (VN173, VC155) that become fluorescent when they reassemble into full fluorescent protein. Thus, it is useful for protein-protein interactions studies involving bimolecular fluorescence complementation approach (BiFC) (PMID: 16454041, 18846096). Here, an improved version of VN173 fragment is used (I152L) to inhibit self-assembly (PMID:21091444). Venus fragments coding sequences were cloned from Addgene ID: 22010 and 22011 and VN173 was subjected to site-directed mutagenesis to introduce mutation I152L.

Proper citation: RRID:Addgene_105804 Copy   

•   Source: Addgene

http://www.addgene.org/105802

Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK-BI16 series which belongs to the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system. ORF2 is cloned with the universal SLIC protocol (just three universal PCR primers are required to clone a given coding sequence into all vectors from pKK family by a ligation-independent DNA cloning method), ORF1 can be cloned using SLIC with specifically designed primers (applicable enzymes: Bsp120I, ApaI, MluI, NotI, BspTI, Eco47III, MunI) or conventional restriction-ligase based cloning (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101). mClover3 is a brighter derivative of mEGFP. mRuby3 is brighter than mCherry and has slightly shifted excitation and emission spectra. The mClover3-mRuby3 pair is superior to EGFP-mCherry in FRET analysis (PMID: 26879144). Useful for experiments requiring co-expression of two proteins (e.g. FRET measurements).

Proper citation: RRID:Addgene_105802 Copy   

•   Source: Addgene

http://www.addgene.org/105922

Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Entire transgene cassette can be removed with NotI/XbaI digest; CBh promoter can be removed by XbaI/AgeI digest; mKate2 can be removed with SaI/EcoRI digest; IRES can be removed by EcoRI/FseI digest

Proper citation: RRID:Addgene_105922 Copy   

•   Source: Addgene

http://www.addgene.org/105865

Species: Synthetic
Genetic Insert: K-GECO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338710
Comments: bioRxiv--A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein (https://doi.org/10.1101/213082)

Proper citation: RRID:Addgene_105865 Copy   

•   Source: Addgene

http://www.addgene.org/105799

Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. CyOFP1 has large Stokes shift. It is excited in the blue and emits in the orange range. It can be multiplexed with EGFP in fluorescence based assays. Also as acceptor for blue-emitting luciferase (PMID: 27240196). CyOFP1 coding sequence was cloned from Addgene ID 74279.

Proper citation: RRID:Addgene_105799 Copy   

•   Source: Addgene

http://www.addgene.org/105840

Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488
Comments: Please note that this plasmid may runs as a dimer (>21kb). Concatenation/Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_105840 Copy   

•   Source: Addgene

http://www.addgene.org/105842

Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488

Proper citation: RRID:Addgene_105842 Copy   

•   Source: Addgene

http://www.addgene.org/105948

Species: Homo sapiens
Genetic Insert: EB3
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237

Proper citation: RRID:Addgene_105948 Copy   

•   Source: Addgene

http://www.addgene.org/105942

Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237

Proper citation: RRID:Addgene_105942 Copy   

•   Source: Addgene

http://www.addgene.org/105998

Species: Staphylococcus aureus
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Addgene NGS results show a C-terminal HA tag rather than 3x HA tag. Also, the mCD4 selection marker matches NCBI reference NP_038516.1 and contains residues 1-427. Plasmids should function as described in the associated publication.

Proper citation: RRID:Addgene_105998 Copy   

•   Source: Addgene

http://www.addgene.org/105997

Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR, S.pyogenes sgRNA expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Dual sgRNA vector to express one sgRNA for S.pyogenes Cas9 and one sgRNA for S.aureus Cas9. Cloning is done in two steps: 1.) AarI digest to clone the S.pyogenes sgRNA and after preparation of the successfully cloned plasmid 2.) BfuAI digest followed by cloning of the S.aureus sgRNA.

Proper citation: RRID:Addgene_105997 Copy   

•   Source: Addgene