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Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113763 Copy
Species: E. coli
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:psgRNA; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_114006 Copy
Species: E. coli
Genetic Insert: clbI
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114151 Copy
Species: E. coli
Genetic Insert: clbG
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114150 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114156 Copy
Species: E. coli
Genetic Insert: clbI
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114159 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114158 Copy
Species: E. coli
Genetic Insert: clbC
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114145 Copy
Species: E. coli
Genetic Insert: clbH-A1
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114149 Copy
Species: E. coli
Genetic Insert: Polyphosphate Kinase 1
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590613
Proper citation: RRID:Addgene_108850 Copy
Species: E. coli
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118253 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118261 Copy
Species: E. coli
Genetic Insert: MetN (N295A)
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118260 Copy
Species: E. coli
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30352853
Proper citation: RRID:Addgene_118581 Copy
Species: E. Coli
Genetic Insert: birA
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:10682; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30639242
Proper citation: RRID:Addgene_120558 Copy
Species: E. Coli
Genetic Insert: BioID
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30220460
Proper citation: RRID:Addgene_121046 Copy
Species: E. Coli
Genetic Insert: BioID
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30220460
Proper citation: RRID:Addgene_121049 Copy
Species: E. coli
Genetic Insert: Protein A-Tnp
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31431618
Comments: There is a single bp deletion in LacI that changes the C terminal coding sequence to FPDWKAGSERNAINVS. This change does not affect function.
Please visit https://doi.org/10.1101/571208 for bioRxiv preprint.
Proper citation: RRID:Addgene_121137 Copy
Species: E. coli
Genetic Insert: LACZ
Vector Backbone Description: Backbone Size:5112; Vector Backbone:pRS303:GAL1-10; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1169 Copy
Species: E. coli
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8318; Vector Backbone:pXPR_BRD003; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30860482
Proper citation: RRID:Addgene_117072 Copy
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