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Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11719502
Comments: Addgene has found that this plasmid is extremely slow growing. We recommend keeping plates and liquid cultures growing for at least 20 hours. Overnight will not be sufficient.
There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.
Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_12212 Copy
Species: Synthetic
Genetic Insert: acrosin-GFP-GPI
Vector Backbone Description: Backbone Marker:Carmen Williams; Backbone Size:3059; Vector Backbone:pIVT2; Vector Types:For in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26623518
Proper citation: RRID:Addgene_122141 Copy
Species: Mus musculus
Genetic Insert: Smarca4
Vector Backbone Description: Backbone Marker:Carmen Williams; Backbone Size:3059; Vector Backbone:pIVT2; Vector Types:For in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29325037
Proper citation: RRID:Addgene_122146 Copy
Genetic Insert: pRas189 Gly12
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCT-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12218 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122093 Copy
Species: Synthetic
Genetic Insert: rsCaMPARI
Vector Backbone Description: Backbone Size:4270; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32931424
Proper citation: RRID:Addgene_122092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI restriction endonuclease
Vector Backbone Description: Backbone Marker:de Lorenzo´s lab; Backbone Size:3224; Vector Backbone:pSEVA121; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122095 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:de Lorenzo´s lab; Vector Backbone:pSEVA448; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122096 Copy
Species: Synthetic
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122099 Copy
Genetic Insert: dCas9-10X GCN4-P2A-mCherry
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318302
Proper citation: RRID:Addgene_122132 Copy
Species: Synthetic
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122098 Copy
Genetic Insert: B. subtilis Rex
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122134 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122135 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122138 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122137 Copy
Species: Synthetic
Genetic Insert: NmCas9 tracrRNA
Vector Backbone Description: Backbone Size:4314; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122091 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: SpATG101
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976798
Proper citation: RRID:Addgene_122081 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-TERF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122084 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-LaminB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122083 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122086 Copy
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