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Species: Homo sapiens
Genetic Insert: RUNX2(+10A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145268 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5B B56 beta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Addgene NGS identified an I346V mutation in the B56β ORF, this is not predicted to impact plasmid function.
Proper citation: RRID:Addgene_14533 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724397.2. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145389 Copy
Species: Homo sapiens
Genetic Insert: TBP(38Q)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145269 Copy
Species: Homo sapiens
Genetic Insert: HOXA13(+7A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145266 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 2'-O-ribose methyltransferase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725311.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145387 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5A B56 alpha regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14532 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145384 Copy
Species: Homo sapiens
Genetic Insert: HOXD13wt-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145260 Copy
Species: Homo sapiens
Genetic Insert: HOXD13(+7A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145382 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Ala)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14539 Copy
Species: Mus musculus
Genetic Insert: mouse SMC3
Vector Backbone Description: Vector Backbone:pKS037-pCAGGS-3XFLAG-Smc3-mKate2-bpAk; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Proper citation: RRID:Addgene_156447 Copy
Species: Homo sapiens
Genetic Insert: human CTCF
Vector Backbone Description: Vector Backbone:pKS070-pCAGGS-3XFLAG-(human)CTCF-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Comments: This plasmid has been found to have dimerized. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you still need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_156448 Copy
Species: Mus musculus
Genetic Insert: mouse BORIS
Vector Backbone Description: Vector Backbone:pKS031-pCAGGS-3XFLAG-BORIS-eGFP-bpAk; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Proper citation: RRID:Addgene_156443 Copy
Species: Mus musculus
Genetic Insert: mouse PDS5B
Vector Backbone Description: Vector Backbone:pKS023-pCAGGS-3XFLAG-mKate2-Pds5b-bpAk; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33154377
Proper citation: RRID:Addgene_156442 Copy
Vector Backbone Description: Backbone Size:6921; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.66, Ligation number L3572.
Proper citation: RRID:Addgene_1564 Copy
Species: B. pertussis
Genetic Insert: S20, S16, S1, S15 ribosomal genes
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156359 Copy
Species: N. gonorrhoeae
Genetic Insert: S20, S16 ribosomal genes
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156358 Copy
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