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Species: Synthetic
Genetic Insert: hCas9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24112467
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original hCas9.
Proper citation: RRID:Addgene_49770 Copy
Species: Synthetic
Genetic Insert: sgRNA_PDS2-Cas9-sgRNA_PDS1
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24112467
Comments: We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition. The construct was assembled using the Golden Gate system from S. Marillonnet.
Proper citation: RRID:Addgene_49772 Copy
Species: Synthetic
Genetic Insert: plamodium falciparum TRAP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50048 Copy
Species: Synthetic
Genetic Insert: pfTRAP(vwa)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50049 Copy
Species: Synthetic
Genetic Insert: G-CaMP3
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23364746
Comments: NOTE: There are some discrepancies between the Addgene quality control sequence and the depositor's full assembled sequence. These differences are not in functionally relevant parts of the plasmid.
Proper citation: RRID:Addgene_50022 Copy
Species: Synthetic
Genetic Insert: Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
Vector Backbone Description: Backbone Size:4086; Vector Backbone:pCS2+; Vector Types:Multi-purpose expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50020 Copy
Species: Synthetic
Genetic Insert: Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs
Vector Backbone Description: Vector Backbone:Lab made; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50347 Copy
Species: Synthetic
Genetic Insert: Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs
Vector Backbone Description: Vector Backbone:Lab made; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50346 Copy
Species: Synthetic
Genetic Insert: SNAP
Vector Backbone Description: Backbone Size:5388; Vector Backbone:pCDNA3.1(+)-myc-GP135; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23615438
Proper citation: RRID:Addgene_50376 Copy
Species: Synthetic
Genetic Insert: CLIP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-puro-FLAG-CD59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23615438
Proper citation: RRID:Addgene_50375 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-FLAG-CD59-GPI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Proper citation: RRID:Addgene_50378 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-puro-FLAG-CD59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Comments: Please click View Sequences link for full plasmid sequence of vector backbone pME-puro-FLAG-CD59.
Proper citation: RRID:Addgene_50377 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:3296; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24608181
Comments: Note: Addgene's quality control sequencing found a few mismatches compared to the depositor's full sequence, but they should not affect plasmid function.
Proper citation: RRID:Addgene_50549 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
Species: Synthetic
Genetic Insert: Luciferase-let7-Poly(A)
Vector Backbone Description: Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAHW; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23123195
Comments: This plasmid expresses a Renilla luciferase reporter for let-7 miRNA with a regular polyadenylation signal.
Proper citation: RRID:Addgene_50556 Copy
Species: Synthetic
Genetic Insert: DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRS426 Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11000112
Comments: There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function.
Proper citation: RRID:Addgene_50572 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50582 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50580 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50587 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50589 Copy
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