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Species: Saccharomyces cerevisiae
Genetic Insert: Ste7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS311; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8062390
Comments: Lex-Ste7C has the 1 kb BglII-BamHI fragment of pYEE129 at BamHI of pYBS311
Proper citation: RRID:Addgene_18886 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast ubiquitin conjugating enzyme Mms2
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Comments: Hofmann R.M. Pickart C.M. 1999, Cell 96, 645-653
Proper citation: RRID:Addgene_18893 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin conjugating enzyme UBC13
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Proper citation: RRID:Addgene_18894 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin C-terminal hydrolase I
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Comments: Johnston SC, Riddle SM, Cohen RE, Hill CP. EMBO J. 1999 Jul 15;18(14):3877-87
Proper citation: RRID:Addgene_18895 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc28
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS311; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Also called LexA-Cdc28
Proper citation: RRID:Addgene_18901 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS21
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT6
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18845 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GOS1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18848 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CHC1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase
Vector Backbone Description: Backbone Marker:Guzman et al.; Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_19041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG18
Vector Backbone Description: Backbone Size:6112; Vector Backbone:Ycplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Comments: ATG18 was cloned with 300bp upstream and downstream of the gene. The myc tag was added later by mutagenesis.
Proper citation: RRID:Addgene_42530 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsv2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHIS2 parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Proper citation: RRID:Addgene_42524 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kex2 protease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5140; Vector Backbone:pcDNA6/V5-His Version C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23205847
Proper citation: RRID:Addgene_42504 Copy
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