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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V
 
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RRID:Addgene_147805 HsDCP1a-S12AQ18AH19A Homo sapiens Kanamycin PMID:24510189 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin two silent mutations T237C and T1716G compared to the sequence given by NCBI AY146651.1. 2026-09-19 02:10:39 0
pRD182 (Roc1b)
 
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RRID:Addgene_14823 Roc1b Drosophila melanogaster Ampicillin Backbone Marker:Promega; Backbone Size:2600; Vector Backbone:T7 IVT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
pRD183 (Roc2)
 
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RRID:Addgene_14824 Roc2 Drosophila melanogaster Ampicillin Backbone Marker:Promega; Backbone Size:2600; Vector Backbone:T7 IVT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
pnYC-NpM-HsNot1iso1_1356-1581_V
 
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RRID:Addgene_147779 HsNot1iso1_1356-1581 Homo sapiens Chloramphenicol PMID:24768540 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-09-19 02:10:39 0
pcDNA3.1 Hmga2 wt
 
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1+ mentions
RRID:Addgene_14789 Hmga2 wt Mus musculus Ampicillin PMID:17322030 The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen). Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 3
pRD119 (PCNA GFP)
 
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RRID:Addgene_14822 PCNA-GFP Drosophila melanogaster Ampicillin PMID:12526745 PCNA GFP fusion (glow); Asp718-NotI fragment from pRD113 cloned into pCasper4IN; GFP fused in-frame using 5' blunt NheI and 3' XbaI (replaces PCNA coding to STOP [XbaI] but has PCNA 3'; GFP from pNEGFPX.1. Backbone Marker:na; Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
pRD60 (dE2F)
 
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RRID:Addgene_14820 E2F Drosophila melanogaster Ampicillin Dros14 dE2F clone flipped at EcoRI site; XhoI digestion gives small fragment--i.e. 5' end near KpnI site; contains entire 3' UTR from Genebank sequence including polyA. Backbone Marker:na; Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
pAc5.1B-lambdaN-HA-DmSKI8_AA
 
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RRID:Addgene_148227 DmSKI8 Drosophila melanogaster Ampicillin sequence given by NCBI:NM_141712.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
MSCV let-7g
 
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1+ mentions
RRID:Addgene_14784 let-7g Mus musculus Ampicillin PMID:17401365 miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: let-7g: 5’-UGAGG UAGUA GUUUG UACAG U-3’. Backbone Marker:BD Biosciences; Backbone Size:6500; Vector Backbone:MSCV-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 1
Hmga2 3'UTR wt luciferase (Luc-wt)
 
Resource Report
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1+ mentions
RRID:Addgene_14785 Luc-wt Mus musculus Ampicillin PMID:17322030 The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). To construct the Renilla luciferase reporters, wild-type and mutant Hmga2 3' UTRs were amplified (PCR primers, 5'-GCGTCTCGAGGGGCGCCGACATTC and 5'GGCGCGGCCGCAGTCAGAGGGCACAC) and cloned into the XbaI and NotI sites of pIS1. Backbone Marker:Available from Addgene (#12179); Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 8
pnYC-NpM-NcPAN2_1-390_Y
 
Resource Report
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RRID:Addgene_148103 NcPAN2_1-390 Neurospora crassa Chloramphenicol PMID:24880343 ORF extracted from N. crassa Databank NCU10733.7 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-09-19 02:10:40 0
pSuper mouse Drosha1
 
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RRID:Addgene_14780 Drosha shRNA Mus musculus Ampicillin PMID:17401365 shRNA sequence: GATCCCCCAA CAGTCATAGA ATATGATTCA AGAGATCATA TTCTATGACT GTTGTTTTTG GAAC Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-09-19 02:10:39 0
pSicoR mouse Dicer3
 
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RRID:Addgene_14781 Dicer shRNA Mus musculus Ampicillin PMID:17401365 shRNA sequence: TGCATGGTGG TGTCGATATT TTCAAGAGAA ATATCGACAC CACCATGCTT TTTTC Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-19 02:10:39 0
pT7-EGFP-C1-HsNot1iso1_1-1840-shRNAres_W
 
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RRID:Addgene_147891 HsNot1iso1_1-1840-shRNAres Homo sapiens Kanamycin PMID:24768540 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 1 silent mutation compared to the sequence of isoform 1 given by NCBI: NM_016284.4. 2026-09-19 02:10:40 0
pcDNA3.1-MS2-HA-HsSmg5-G120E_W
 
Resource Report
Resource Website
RRID:Addgene_147819 HsSmg5-G120E Homo sapiens Ampicillin PMID:24115769 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin one silent mutation compared to the sequence given by NCBI: NM_015327.2. 2026-09-19 02:10:39 0
pRK Smad3NL Flag
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14828 Smad3 NL Homo sapiens Ampicillin PMID:9094310 See Author's map for cloning details. Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin amino acids 1-210 2026-09-19 02:10:40 1
pcDNA3.1-MS2-HA-HsNanos3-del4-20_X
 
Resource Report
Resource Website
RRID:Addgene_147945 HsNanos3-del4-20 Homo sapiens Ampicillin PMID:24736845 synthesized DNA from GeneArt. Codon optimized for Expression in human cells. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 0
pcDNA3/HA-RBP2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14799 Retinoblastoma Binding Protein 2 Homo sapiens Ampicillin PMID:17320163 The plasmid encodes isoform 2. Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:40 3
pRK Smad3C Flag
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14830 Smad3 C Homo sapiens Ampicillin PMID:9094310 See Author's map for cloning details. Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin amino acids 199-424 2026-09-19 02:10:40 1
pRK5 TGF beta type I receptor Flag
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14831 TGF beta type I receptor Rattus norvegicus Ampicillin PMID:8662796 See Author's map for more information. A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function. Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:10:41 4

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