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Species: Homo sapiens
Genetic Insert: Rab5C
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166426 Copy
Species: Synthetic
Genetic Insert: SCW-HHL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693235
Comments: Please visit https://doi.org/10.1101/2022.06.20.496847 for bioRxiv preprint
Proper citation: RRID:Addgene_190634 Copy
Species: Drosophila melanogaster
Genetic Insert: Dorado
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190638 Copy
Species: Homo sapiens
Genetic Insert: optineurin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pET Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29343546
Comments: Link to ASAP Hub: https://hub.asap.science/shared-research/c7069697-8e31-46da-8d5a-9af4c3777f30
Proper citation: RRID:Addgene_190192 Copy
Species: Homo sapiens
Genetic Insert: TrkB
Vector Backbone Description: Backbone Marker:Forough R, et al. J Biol Chem. 1993 Feb 5;268(4):2960-8. PMID: 7679105; Backbone Size:6366; Vector Backbone:pMEXneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9488719
Proper citation: RRID:Addgene_190203 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36202974
Proper citation: RRID:Addgene_183231 Copy
Species: Drosophila melanogaster
Genetic Insert: Dorado
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190608 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169170 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169171 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Vector Backbone:pRS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_190453 Copy
Species: Homo sapiens
Genetic Insert: ZMYND8
Vector Backbone Description: Vector Backbone:LentiV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34358447
Proper citation: RRID:Addgene_190619 Copy
Species: Synthetic
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Addgene #60847; Vector Backbone:pCAS; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36287051
Comments: This plasmid is a modified version of pCAS (Addgene #60847) that has been improved to facilitate rapid cloning of new gRNA sequencing for streamlined CRISPR-Cas9 genome editing in yeast. pCAS was originally published by:
Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
Proper citation: RRID:Addgene_190175 Copy
Species: Homo sapiens
Genetic Insert: CDC42BPA
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189907 Copy
Species: Homo sapiens
Genetic Insert: ADCK2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189908 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Thermo; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35903559
Proper citation: RRID:Addgene_189675 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Thermo; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35903559
Proper citation: RRID:Addgene_189676 Copy
Species: Drosophila melanogaster
Genetic Insert: dora (CG34401) sgRNA 1
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:10615; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190700 Copy
Species: Drosophila melanogaster
Genetic Insert: dora (CG34401) sgRNA 2
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:10615; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190701 Copy
Species: Staphylococcus aureus
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36162812
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_188504 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Thermo; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35903559
Proper citation: RRID:Addgene_189722 Copy
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