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Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990037
Proper citation: RRID:Addgene_1117 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Marker:Cell biolabs; Backbone Size:5900; Vector Backbone:pMx-puromycin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25416133
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111809 Copy
Species: Homo sapiens
Genetic Insert: DOT1L
Vector Backbone Description: Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29563185
Proper citation: RRID:Addgene_111755 Copy
Genetic Insert: EYFP from Aequorea victoria
Vector Backbone Description: Backbone Marker:Available at Addgene (#11160); Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14603031
Proper citation: RRID:Addgene_11180 Copy
Species: Homo sapiens
Genetic Insert: DOT1L
Vector Backbone Description: Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29563185
Proper citation: RRID:Addgene_111756 Copy
Species: Homo sapiens
Genetic Insert: MLLT10
Vector Backbone Description: Backbone Marker:Arrowsmith lab (Addgene plasmid# 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29563185
Comments: N-Tag:MHHHHHHSSGRENLYFQG. MLLT10:JMC076-B05:C227962.
SGC link: http://www.thesgc.org/structures/6CKN/
Proper citation: RRID:Addgene_111753 Copy
Species: Homo sapiens
Genetic Insert: DOT1L
Vector Backbone Description: Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29563185
Proper citation: RRID:Addgene_111754 Copy
Species: Synthetic
Genetic Insert: 2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
Vector Backbone Description: Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30315152
Proper citation: RRID:Addgene_111759 Copy
Species: Synthetic
Genetic Insert: 2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
Vector Backbone Description: Vector Backbone:β-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30315152
Proper citation: RRID:Addgene_111758 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111741 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111745 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111742 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111748 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111746 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111747 Copy
Species: Homo sapiens
Genetic Insert: TBG1
Vector Backbone Description: Backbone Marker:Markku Varjosalo, Addgene plasmid #108078; Vector Backbone:MAC-tag-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29568061
Comments: CCSB-DFCI hORFeome collection(11037_B03)
Proper citation: RRID:Addgene_111696 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111730 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Please screen multiple clones and validate plasmid by sequencing or restriction analysis before proceeding with downstream experiments
Proper citation: RRID:Addgene_111733 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111734 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111698 Copy
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