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Species: Mus musculus
Genetic Insert: sgNT3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35228570
Proper citation: RRID:Addgene_177239 Copy
Species: Prevotella buccae
Genetic Insert: single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35649413
Proper citation: RRID:Addgene_184839 Copy
Vector Backbone Description: Backbone Size:5577; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal mScarlet-I tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186383 Copy
Species: Synthetic
Genetic Insert: PR-Egfp
Vector Backbone Description: Backbone Marker:expressys.; Backbone Size:1720; Vector Backbone:Pz; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32781386
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.26.920629v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185973 Copy
Species: Synthetic
Genetic Insert: Pl-LacO1-Egfp
Vector Backbone Description: Backbone Marker:expressys.; Backbone Size:1720; Vector Backbone:Pz; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32781386
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.26.920629v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185971 Copy
Vector Backbone Description: Backbone Size:5583; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal mClover3 tag at EcoR V/Avr II restriction site
Proper citation: RRID:Addgene_186389 Copy
Species: Homo sapiens
Genetic Insert: beta2M (MHC Class I light)
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187357 Copy
Species: Homo sapiens
Genetic Insert: hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187356 Copy
Species: Homo sapiens
Genetic Insert: Truncated hCD3 delta, and GFP2
Vector Backbone Description: Vector Backbone:pHRSinGFP2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187355 Copy
Vector Backbone Description: Backbone Size:5796; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal emiRFP703 tag at EcoR V/Avr II restriction site
Proper citation: RRID:Addgene_186386 Copy
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal Snap-tag tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186385 Copy
Vector Backbone Description: Backbone Size:5598; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal mVenus tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186384 Copy
Species: Synthetic
Genetic Insert: PlTetO-1 AntitdTomatosynsrRNA
Vector Backbone Description: Backbone Marker:expressys.; Backbone Size:2112; Vector Backbone:Pz; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32781386
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.26.920629v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185975 Copy
Species: E. coli
Genetic Insert: nuoEFG
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35814529
Proper citation: RRID:Addgene_187087 Copy
Species: E. coli
Genetic Insert: nuoA-N
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35814529
Proper citation: RRID:Addgene_187086 Copy
Species: Homo sapiens
Genetic Insert: GPa3b17 hTCR beta
Vector Backbone Description: Vector Backbone:pHRSin; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187361 Copy
Vector Backbone Description: Backbone Size:5574; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal mNeonGreen tag at EcoR V/Avr II restriction site
Proper citation: RRID:Addgene_186392 Copy
Species: E. coli
Genetic Insert: nuoA-N
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35814529
Proper citation: RRID:Addgene_187084 Copy
Vector Backbone Description: Backbone Size:5547; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal mEos4b tag at EcoR V/Avr II restriction site
Proper citation: RRID:Addgene_186390 Copy
Species: Synthetic
Genetic Insert: pooled library
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187248 Copy
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