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Vector Backbone Description: Vector Backbone:pLKO-Cre stuffer v3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32165588
Proper citation: RRID:Addgene_158032 Copy
Vector Backbone Description: Vector Backbone:pLKO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32165588
Proper citation: RRID:Addgene_158030 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:PLX306 iCre; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32165588
Proper citation: RRID:Addgene_158050 Copy
Species: Synthetic
Genetic Insert: mGold-P2A-EBFP2
Vector Backbone Description: Backbone Size:6992; Vector Backbone:pJL2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: A mammalian expression cassette was cloned into a yeast vector.
mGold: FPBaseID TBD
EBFP2: FPBaseID DVMQ7 Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157998 Copy
Species: Synthetic
Genetic Insert: mGold
Vector Backbone Description: Backbone Size:6950; Vector Backbone:pJL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157997 Copy
Species: Synthetic
Genetic Insert: CloGFP(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33227014
Proper citation: RRID:Addgene_157994 Copy
Species: Synthetic
Genetic Insert: pSFFV_mNG3K(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33227014
Proper citation: RRID:Addgene_157993 Copy
Species: Synthetic
Genetic Insert: mGold
Vector Backbone Description: Backbone Size:6105; Vector Backbone:pCaggs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33097540
Comments: Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_157996 Copy
Species: Homo sapiens
Genetic Insert: Spleen tyrosine kinase
Vector Backbone Description: Vector Backbone:pVL1393; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29890824
Proper citation: RRID:Addgene_157990 Copy
Species: Mus musculus
Genetic Insert: Adam10 sgRNA
Vector Backbone Description: Vector Backbone:pLKO-ERT2 Cre stuffer v3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32165588
Proper citation: RRID:Addgene_158047 Copy
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBSII-KS(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33016195
Proper citation: RRID:Addgene_157991 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein optimised for excitation with UV light
Vector Backbone Description: Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33226934
Proper citation: RRID:Addgene_158060 Copy
Species: Escherichia coli
Genetic Insert: lacIZ operon
Vector Backbone Description: Backbone Marker:Addgene #158058; Backbone Size:8079; Vector Backbone:pDOC-K-glmS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33226934
Proper citation: RRID:Addgene_158061 Copy
Species: Salmonella enterica Ser. Typhimurium
Genetic Insert: glmS homologous region 1
Vector Backbone Description: Backbone Size:7233; Vector Backbone:pDOC-K; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33226934
Proper citation: RRID:Addgene_158058 Copy
Species: Homo sapiens
Genetic Insert: PHKG1-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_158192 Copy
Species: Homo sapiens
Genetic Insert: DYRK3-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: Depositor confirms R293H does not affect plasmid function. This is a known sequence conflict.
These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_158190 Copy
Species: Synthetic
Genetic Insert: 5'UTR synthetic stem-loop -30kcal/mol
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32846140
Proper citation: RRID:Addgene_158103 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR of CACNA1C (uc009zdu.1)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32846140
Proper citation: RRID:Addgene_158104 Copy
Species: Homo sapiens
Genetic Insert: BCKDK-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_158189 Copy
Species: Synthetic
Genetic Insert: 5'UTR synthetic stem-loop 0kcal/mol
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4716; Vector Backbone:pGL4.53(luc2/PGK); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32846140
Proper citation: RRID:Addgene_158102 Copy
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