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Genetic Insert: epegRNA-eBAR3
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240537 Copy
Genetic Insert: epegRNA-eBAR2
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240536 Copy
Species: Synthetic
Genetic Insert: attR1-CmR-ccdB-attR2
Vector Backbone Description: Vector Backbone:pB-TA-ERN; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242578 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:13560; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a Gal4 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. w uniform transghenes expression. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173726 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:6943; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225282 Copy
Vector Backbone Description: Backbone Size:6238; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225281 Copy
Vector Backbone Description: Backbone Size:6943; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225280 Copy
Species: Synthetic
Genetic Insert: attR1-CmR-ccdB-attR2
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242574 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79469 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79466 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79476 Copy
Species: Mus musculus
Genetic Insert: Foxo1-WT-DBD
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pDNR-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17717603
Comments: This plasmid also contains a E226G mutation--the author has determined that this mutation does not affect the plasmid detrimentally. It also has K219R and L619P polymorphisms.
Proper citation: RRID:Addgene_17555 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141013 Copy
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://doi.org/10.1101/2022.12.13.520107 for bioRxiv preprint.
Proper citation: RRID:Addgene_195985 Copy
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://doi.org/10.1101/2022.12.13.520107 for bioRxiv preprint.
Proper citation: RRID:Addgene_195989 Copy
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://doi.org/10.1101/2022.12.13.520107 for bioRxiv preprint.
Proper citation: RRID:Addgene_195983 Copy
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42424437
Comments: pHAGE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Proper citation: RRID:Addgene_259769 Copy
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42424437
Comments: pHAGE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Proper citation: RRID:Addgene_259774 Copy
Vector Backbone Description: Backbone Marker:Soriano Lab (Addgene Plasmid# 21714); Backbone Size:9846; Vector Backbone:pRosa26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21375737
Comments: Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette.
Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418
Proper citation: RRID:Addgene_24418 Copy
Species: reporter gene eGFP
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10444; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24566 Copy
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