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Species: Synthetic
Genetic Insert: S(E2F1)p-mKate2
Vector Backbone Description: Backbone Size:9000; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056342
Proper citation: RRID:Addgene_105177 Copy
Species: HIV-1
Genetic Insert: Vpr
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29046351
Comments: The pMM310-BlamVpr plasmid reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pMM310(Cat#11444) from Dr. Michael Miller. The DNA sequences encoding for Beta-lactamase (Blam) was replaced with HindIII-mCherry-PC-NotI-PC-YFP-BamHI encoding sequences.
mCherry-pc2x-eYFPVpr plasmid reference:
Sood C, Francis AC, Desai TM, Melikyan GB.An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.J Biol Chem. 2017 Oct 18. pii: jbc.M117.818088. doi: 10.1074/jbc.M117.818088.
pMM310 plasmid reference:
Tobiume M, Lineberger JE, Lundquist CA, Miller MD, and Aiken C. Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells. J. Virology 77(19); 10645-50, 2003. [Abstract].
An in-depth description of a similar system: Cavrois M, De Noronha C, Greene WC. A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes. Nat Biotechnol. 2002 Nov;20(11):1151-4. Epub 2002 Sep 30. [Abstract]
Proper citation: RRID:Addgene_105215 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Vector Backbone:pEPet-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22547408
Comments: Subcloned the sequence encoding amino acids 65-150 (with an additional nucleotide (C) added at the 5' end to keep it in frame) of human Pak1 into the EcoRI-BamH1 sites of pYPet-C1. The insert was from Dr. Natalie Lamarche-Vane (McGill University).
Proper citation: RRID:Addgene_105290 Copy
Species: Synthetic
Genetic Insert: G8p-antiPD1(HC)-P2A-antiPD1(LC)
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056342
Proper citation: RRID:Addgene_105200 Copy
Species: Homo sapiens
Genetic Insert: Tensin3
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20069572
Comments: Note: The TNS3 insert contains a C1276C mutation compared to the listed reference sequence. The depositor has confirmed it does not affect plasmid function.
Proper citation: RRID:Addgene_105299 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7c variant 1513
Vector Backbone Description: Backbone Size:4579; Vector Backbone:AAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31209382
Proper citation: RRID:Addgene_105321 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7c variant 1513
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31209382
Proper citation: RRID:Addgene_105320 Copy
Species: C. difficile / synthetic
Genetic Insert: SmBiT/HupA-LgBiT
Vector Backbone Description: Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30633871
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint.
Proper citation: RRID:Addgene_105497 Copy
Species: C. difficile / synthetic
Genetic Insert: HupA-SmBiT/LgBiT
Vector Backbone Description: Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30633871
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint.
Proper citation: RRID:Addgene_105496 Copy
Species: Mus musculus
Genetic Insert: Trpc2
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-Teasy; Vector Types:to ganerate riboprobe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25001287
Comments: Linearize with SpeI, use T7 RNA polymerase for IVT.
Proper citation: RRID:Addgene_105473 Copy
Species: Mus musculus
Genetic Insert: Trpc2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:to ganerate riboprobe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25001287
Comments: Linearize with SpeI, use T7 RNA polymerase for IVT.
Proper citation: RRID:Addgene_105474 Copy
Species: Mus musculus
Genetic Insert: TRIM21
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4697; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29153837
Proper citation: RRID:Addgene_105518 Copy
Species: Mus musculus
Genetic Insert: TRIM21
Vector Backbone Description: Backbone Size:3816; Vector Backbone:pGEMHE; Vector Types:in vitro Transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29153837
Comments: The sequence contains an E318K in mTrim21. This mutation does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_105519 Copy
Species: Mus musculus
Genetic Insert: TRIM21
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4694; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29153837
Comments: The sequence contains an E318K in mTrim21. This mutation does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_105516 Copy
Species: Mus musculus
Genetic Insert: TRIM21
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4692; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29153837
Comments: The sequence contains an E318K in mTrim21. This mutation does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_105517 Copy
Species: Mus musculus
Genetic Insert: TRIM21
Vector Backbone Description: Backbone Size:3816; Vector Backbone:pGEMHE; Vector Types:in vitro Transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29153837
Comments: The sequence contains an E318K in mTrim21. This mutation does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_105520 Copy
Vector Backbone Description: Backbone Size:7795; Vector Backbone:MSCV-Neo-IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757253
Proper citation: RRID:Addgene_105505 Copy
Genetic Insert: Chronos-mRuby2-ST
Vector Backbone Description: Backbone Size:5855; Vector Backbone:pCAGGS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29089483
Proper citation: RRID:Addgene_105446 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Luciferase; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core number p0105
Proper citation: RRID:Addgene_105532 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Luciferase; Bacterial Resistance:Ampicillin
Comments: Penn Vector Core number p0109
Proper citation: RRID:Addgene_105533 Copy
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