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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA5-FRT-TO-Nsp16 Resource Report Resource Website |
RRID:Addgene_157723 | Nsp16 | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-Nsp15-HF Resource Report Resource Website |
RRID:Addgene_157722 | Nsp15-HF | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-Nsp16-HF Resource Report Resource Website |
RRID:Addgene_157725 | Nsp16-HF | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-FH-Nsp16 Resource Report Resource Website |
RRID:Addgene_157724 | FH-Nsp16 | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-FH-Nsp15 Resource Report Resource Website |
RRID:Addgene_157721 | FH-Nsp15 | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-Nsp15 Resource Report Resource Website |
RRID:Addgene_157720 | Nsp15 | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized | 2026-09-19 02:11:43 | 0 | |
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pMBPcyto-TEV-BseRI Resource Report Resource Website |
RRID:Addgene_157739 | Ampicillin | - Empty vector for cytoplasmic expression of N-terminally His10+MBP-tagged protein of interest, where tag removal by TEV protease yields the native sequence for the protein of interest. - Based on plasmid pET PPL His6 MBP LIC cloning vector (2K-T) (Plasmid #37183), but has the added advantage in leaving no added amino acid residues following cleavage with TEV protease. This advantage is made possible by cloning into the two BseRI restriction sites. - Subcloning requires ligation-independent cloning and use of the two BseRI restriction sites that are present in this empty vector. The two BseRI sites span a removable 432 nucleotide sequence. Cloning into the BseRI-digested vector can be achieved using a ligation-independent system that is dependent on homologous recombination, such as the Takara In-Fusion HD Cloning Plus system, which requires simply designing the PCR-derived insert (containing the protein of interest) to contain 15 bp at each end that matches the 15 bp at each end of the linearized parent vector. In other words, add these 15 bp, AACCTGTACTTCCAA, to the 5' end of the PCR FORWARD primer, and add these 15 bp, CGCGATCGCGGATCC, to the 5' end of the PCR REVERSE primer. - The sequence of the added N-terminus is MKSS + His10 + GSSM + maltose binding protein (aa K27-T392 of NP_290668) + NSSSNNNNNNNNNNLGIE + ENLYFQ*X, where * is the TEV protease cleavage site and X is the N-terminal residue of the introduced protein of interest. Note that TEV protease cleaves most efficiently when X = S, G, A, M; for other compatible residues see Kapust et al 2002, PMID 12074568. - The maltose binding protein tag is the mature form, without its signal sequence, therefore protein expression is directed to the cytoplasm. - BseRI cuts 8-10 nucleotides outside of its own recognition sequence and is a non-palindromic site. The placement & orientation of the two BseRI sites eliminates all of the BseRI recognition sequence in the resulting subclone. - To verify the PCR-generated insert sequence, use sequencing primers MBPpelBseqF (GAAAGGTGAAATCATGCCGAACATC), and pET-PPLseq1R (CTTCCTTTCGGGCTTTGTTAGC) or T7-Ter (GCTAGTTATTGCTCAGCGG). | Backbone Size:5942; Vector Backbone:pET PPL His6 MBP LIC cloning vector (2K-T) = Addgene #37183; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:43 | 0 | ||||
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pFastBac1-His10FLAG Resource Report Resource Website |
RRID:Addgene_157736 | Ampicillin | PMID:33971243 | - This vector is designed to avoid adding non-native amino acid residues to the protein of interest, following enterokinase cleavage of the N-terminal purification tags (His10 and 3xFLAG). Subcloning requires ligation-independent cloning and use of the two BseRI restriction sites that are present in this empty vector. The two BseRI sites span a removable 432 nucleotide sequence. Cloning into the BseRI-digested vector can be achieved using a ligation-independent system that is dependent on homologous recombination, such as the Takara In-Fusion HD Cloning Plus system, which requires simply designing the PCR-derived insert (containing the protein of interest) to contain 15 bp at each end that matches the 15 bp at each end of the linearized parent vector. In other words, add these 15 bp, GATGACGATGACAAG, to the 5' end of the PCR FORWARD primer, and add these 15 bp, AGTACTTCTCGACAA, to the 5' end of the PCR REVERSE primer. - The sequence of the N-terminus will be MGSS + His10 + DYKDHDGDYKDHDIDYKDDDDK*X, where * is the enterokinase cleavage site and X is the N-terminus of the protein of interest. For X residues that are compatible with enterokinase activity, see Hosfield & Lu 1999, PMID 10094769. - BseRI cuts 8-10 nucleotides outside of its own recognition sequence and is a non-palindromic site. The placement & orientation of the two BseRI sites eliminates all of the BseRI recognition sequence in the resulting subclone. - An unwanted BseRI site was removed from the parent pFastBac1 vector by introducing a silent mutation (CTC to CTG) in a leucine codon in the gentamicin-resistance gene. - To identify colonies containing a cloned insert, used the primers PFASTBAC1F (CTAGTGGTTGGCTACGTATACTCCG) and PFASTBAC1R (GGACAAACCACAACTAGAATGCAGTG). - To verify the PCR-generated insert sequence, use sequencing primers POLYHEDF (AAATGATAACCATCTCGC), SV40PAR (GAAATTTGTGATGCTATTGC). - To verify Tn7-based incorporation of the expression construct into the bacmid DNA in the E. coli strain DH10Bac, use PCR primers BACM13F (CCCAGTCACGACGTTGTAAAACG) and BACM13R (AGCGGATAACAATTTCACACAGG). A bacmid containing the correct insert has a PCR product size of ~2300 base pairs plus the length of the added insert. | Backbone Marker:Invitrogen; Backbone Size:4682; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:43 | 0 | |||
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pcDNA-FRT-TO-Nprot_GC3opt Resource Report Resource Website |
RRID:Addgene_157730 | Nprot_GC3opt | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized; gene expression was optimized by increasing the GC3 content within codons | 2026-09-19 02:11:43 | 0 | |
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pcDNA5-FRT-TO-Nprot-HF_GC3opt Resource Report Resource Website |
RRID:Addgene_157732 | Nprot-HF_GC3opt | SARS-CoV-2 | Ampicillin | PMID:33313418 | Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon optimized; gene expression was optimized by increasing the GC3 content within codons | 2026-09-19 02:11:43 | 0 | |
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pcDNA 3.1 AURKA deltaNter Resource Report Resource Website |
RRID:Addgene_157749 | AURKA | Homo sapiens | Ampicillin | PMID:30070631 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | the first 30AA of Aurora A are lacking | 2026-09-19 02:11:43 | 0 | |
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Plasmid EUPp_09 Resource Report Resource Website |
RRID:Addgene_157745 | acetaldehyde dehydrogenase | Dickeya zeae | Ampicillin | PMID:33248272 | Backbone Size:2502; Vector Backbone:PgyrA_pMB1_ampR; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:43 | 0 | ||
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CaTCH_empty-backbone Resource Report Resource Website 1+ mentions |
RRID:Addgene_157746 | Ampicillin | PMID:32719478 | mCMV_eGFP_EF1as_eBFP2 | Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral, CaTCH barcode cloning; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:43 | 1 | |||
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pelBHisTEV Resource Report Resource Website |
RRID:Addgene_157741 | Kanamycin | - Empty vector (kanamycin-R) adds TEV-protease-removable N-terminal PelB signal sequence + His10, plus potential C-term His6. Non-leaky expression due to T7lac promoter. - Has an advantage in leaving no added amino acid residues following TEV protease cleavage of PelB signal sequence + His10. This advantage is made possible by cloning into the two BseRI restriction sites. - Subcloning requires ligation-independent cloning and use of the two BseRI restriction sites that are present in this empty vector. The two BseRI sites span a removable 432 nucleotide sequence. Cloning into the BseRI-digested vector can be achieved using a ligation-independent system that is dependent on homologous recombination, such as the Takara In-Fusion HD Cloning Plus system, which requires simply designing the PCR-derived insert (containing the protein of interest) to contain 15 bp at each end that matches the 15 bp at each end of the linearized parent vector. In other words, add these 15 bp, AACCTGTACTTCCAA, to the 5' end of the PCR FORWARD primer, and add these 15 bp, GTGGTGGTGCTCGAG, to the 5' end of the PCR REVERSE primer. - The sequence of the added N-terminus is the PelB signal sequence (M1-A22 of GenBank #AAA24848) + 13 aa linker + His10-tag + 4 aa linker + TEV protease cleavage site. The N-terminal protein sequence is: MKYLLPTAAAGLLLLAAQPAMA + MDIGINSDPNSSS + HHHHHHHHHH + PMGS + ENLYFQ*X, where * is the TEV protease cleavage site and X is the N-terminal residue of the introduced protein of interest. Note that TEV protease cleaves most efficiently when X = S, G, A, M; for other compatible residues see Kapust et al 2002, PMID 12074568. - A C-terminal His6-tag is possible: if no stop codon is included in the cloned insert, the sequence LEHHHHHH is added to the C-terminus. - The PelB signal sequence directs the protein of interest to the E. coli inner membrane. Whether the protein traverses the inner membrane is dependent on the protein of interest. - BseRI cuts 8-10 nucleotides outside of its own recognition sequence and is a non-palindromic site. The placement & orientation of the two BseRI sites eliminates all of the BseRI recognition sequence in the resulting subclone. - To verify the PCR-generated insert sequence, use sequencing primers T7 (TAATACGACTCACTATAGGG), T7-Ter (GCTAGTTATTGCTCAGCGG). | Backbone Size:5401; Vector Backbone:pelB-MBP (DNASU access. no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-19 02:11:43 | 0 | ||||
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pEGFP N1 AURKA 1-110 Resource Report Resource Website |
RRID:Addgene_157755 | AURKA | Homo sapiens | Kanamycin | PMID:30070631 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | The first 110 amino acids of AURKA were cloned | 2026-09-19 02:11:43 | 0 | |
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pcDNA 3.1 mitoAURKA Resource Report Resource Website |
RRID:Addgene_157750 | AURKA | Homo sapiens | Ampicillin | PMID:30070631 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Aurora A without the first 30AA + MTS of CoxVIII | 2026-09-19 02:11:43 | 0 | |
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pET19b+H3 Resource Report Resource Website |
RRID:Addgene_157807 | H3 | Homo sapiens | Ampicillin | PMID:31543265 | Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C111A | 2026-09-19 02:11:44 | 0 | |
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pET19b+H2B Resource Report Resource Website |
RRID:Addgene_157809 | H2B | Homo sapiens | Ampicillin | PMID:31543265 | Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:44 | 0 | ||
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pMTTH+eGFP-bromo5 Resource Report Resource Website |
RRID:Addgene_157804 | eGFP-bromo5 | Homo sapiens | Ampicillin | PMID:31543265 | Vector Backbone:modified pMal (MTTH); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:44 | 0 | ||
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pMTTH+bromo5 Resource Report Resource Website |
RRID:Addgene_157803 | bromo5 | Homo sapiens | Ampicillin | PMID:31543265 | Vector Backbone:modified pMal (MTTH); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-19 02:11:44 | 0 |
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