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Species: Mus musculus
Genetic Insert: Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
Vector Backbone Description: Backbone Marker:Addgene Research Program (Addgene plasmid # 242954); Vector Backbone:pAG mKappa Empty; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_242956 Copy
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4244; Vector Backbone:pAG AB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid 242954 pAG mKappa Empty encodes the mouse kappa light chain constant region and a filler sequence in lieu of a light chain variable region. To clone in your desired light chain variable region in frame with the mouse kappa light chain constant region, Addgene recommends the NEB Hifi assembly method.
PCR Amplify the backbone from plasmid 242954 pAG mKappa Empty with the following primers: 5’-cgggctgatgctgcaccaac and 5’-ggccctcgaggacgccagca.
Synthesize a gene fragment of the desired light chain variable region with flanking sequences that overlap with the backbone PCR product 5’-tgctggcgtcctcgagggcc-Gene fragment-cgggctgatgctgcaccaac
Assemble with the NEB HiFI assembly (NEB #E2621S) following the manufacturer’s recommendations
Proper citation: RRID:Addgene_242954 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230120 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230124 Copy
Species: Synthetic
Genetic Insert: Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
Vector Backbone Description: Backbone Marker:IDT; Vector Backbone:pUCIDT-AMP; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits
Proper citation: RRID:Addgene_242204 Copy
Species: Synthetic
Genetic Insert: Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pRP[Exp]; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Further information can be found at https://www.growmorefoundation.org/enable-crispr-kits
Proper citation: RRID:Addgene_242205 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: More information on https://www.growmorefoundation.org/enable-crispr-kits
Please note: This plasmid contains a C nucleotide deletion at P302 in dxs E.coli causing a frameshift and early termination. This deletion does not impact plasmid function.
Proper citation: RRID:Addgene_242207 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243061 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243062 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243060 Copy
Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Iain Fraser, Addgene plasmid #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41148168
Proper citation: RRID:Addgene_232449 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243065 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243064 Copy
Species: Synthetic
Genetic Insert: sgRELA_4
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:pLENTICRISPR V2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_241446 Copy
Species: Synthetic
Genetic Insert: sgmRELA_1
Vector Backbone Description: Backbone Marker:Agata Smogorzewska (Addgene #99154); Vector Backbone:pLENTICRISPR V2-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_241447 Copy
Species: Mus musculus
Genetic Insert: pyruvate kinase, muscle
Vector Backbone Description: Backbone Size:9221; Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41120320
Proper citation: RRID:Addgene_242814 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUTR1-mTurquoise2-2xCrk_SH3
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: Additional inserts: 1) ScYnd1p(1-495)-ePTS1; 2) ScPex22(1-36)-mRuby2-SH3pep
Proper citation: RRID:Addgene_239511 Copy
Species: Synthetic
Genetic Insert: pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
Vector Backbone Description: Vector Backbone:pABE8e-protein (Addgene Plasmid #161788); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40935887
Proper citation: RRID:Addgene_242658 Copy
Species: Homo sapiens
Genetic Insert: PCSK5(M452I)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41148168
Proper citation: RRID:Addgene_232445 Copy
Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Iain Fraser, Addgene plasmid #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:41148168
Proper citation: RRID:Addgene_232448 Copy
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