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Species: Homo sapiens
Genetic Insert: Smad2 (L+MH2)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751102
Proper citation: RRID:Addgene_15730 Copy
Species: Mus musculus
Genetic Insert: p110 delta
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pPyCAG-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12774123
Proper citation: RRID:Addgene_15691 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pHis; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157657 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.109, Ligation number L3609.
Proper citation: RRID:Addgene_1574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP-gRNA
Vector Backbone Description: Backbone Size:1861; Vector Backbone:pTPGI; Vector Types:Insert storage (replicative in E. coli); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33191917
Proper citation: RRID:Addgene_157656 Copy
Vector Backbone Description: Backbone Size:4816; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.111, Ligation number L3610.
Proper citation: RRID:Addgene_1575 Copy
Vector Backbone Description: Backbone Size:6921; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD115.114, Ligation number L3611.
Proper citation: RRID:Addgene_1576 Copy
Vector Backbone Description: Backbone Marker:Arbor Biosciences; Backbone Size:2536; Vector Backbone:pTXTL-P70a(2)-deGFP; Vector Types:Bacterial Expression, Cell-free Expression (E. coli sigma-70); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36427151
Comments: The annotated sequence is also available at the Haynes lab Benchling site: https://benchling.com/s/seq-4Syx826a0gonKiH8Evvg.
Please visit https://doi.org/10.17504/protocols.io.brgcm3sw for the protocol.
Proper citation: RRID:Addgene_157652 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157655 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp10-HF
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157708 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:8800; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157668 Copy
Species: SARS-CoV-2
Genetic Insert: FH-Nsp8-Nsp7_GC3opt
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157701 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp8-Nsp7_GC3opt
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157700 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp9
Vector Backbone Description: Backbone Marker:Thermo Fisher; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313418
Proper citation: RRID:Addgene_157703 Copy
Species: Homo sapiens
Genetic Insert: Histone H2B
Vector Backbone Description: Backbone Marker:Guo et al. 2012 (PMC3478612); Backbone Size:7060; Vector Backbone:pSCMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32579930
Proper citation: RRID:Addgene_157669 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:13000; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157664 Copy
Species: Homo sapiens
Genetic Insert: Fibroblast growth factor 2
Vector Backbone Description: Backbone Size:6377; Vector Backbone:pCAG-HA-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157663 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:8800; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157666 Copy
Species: Homo sapiens
Genetic Insert: Apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:6377; Vector Backbone:pCAG-F-BS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157662 Copy
Species: Homo sapiens
Genetic Insert: Spliceosome RNA helicase DDX39B
Vector Backbone Description: Backbone Size:4900; Vector Backbone:PGEX-4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32383752
Proper citation: RRID:Addgene_157661 Copy
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